Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury

Zhenghui Li; Jin Zhang; Steffen Halbgebauer; Akila Chandrasekar; Rida Rehman; Albert Ludolph; Tobias Boeckers; Markus Huber-Lang; Markus Otto; Francesco Roselli; Florian Olde Heuvel

doi:10.1093/burnst/tkab027

Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury

Zhenghui Li, Jin Zhang, Steffen Halbgebauer, Akila Chandrasekar, Rida Rehman, Albert Ludolph, Tobias Boeckers, Markus Huber-Lang, Markus Otto, Francesco Roselli, Florian Olde Heuvel

Institute of Experimental and Clinical Pharmacology and Toxicology

Abstract

BACKGROUND: Blood-based biomarkers have proven to be a reliable measure of the severity and outcome of traumatic brain injury (TBI) in both murine models and patients. In particular, neuron-specific enolase (NSE), neurofilament light (NFL) and S100 beta (S100B) have been investigated in the clinical setting post-injury. Ethanol intoxication (EI) remains a significant comorbidity in TBI, with 30-40% of patients having a positive blood alcohol concentration post-TBI. The effect of ethanol on blood-based biomarkers for the prognosis and diagnosis of TBI remains unclear. In this study, we investigated the effect of EI on NSE, NFL and S100B and their correlation with blood-brain barrier integrity in a murine model of TBI.

METHODS: We used ultra-sensitive single-molecule array technology and enzyme-linked immunosorbent assay methods to measure NFL, NSE, S100B and claudin-5 concentrations in plasma 3 hours post-TBI.

RESULTS: We showed that NFL, NSE and S100B were increased at 3 hours post-TBI. Interestingly, ethanol blood concentrations showed an inverse correlation with NSE but not with NFL or S100B. Claudin-5 levels were increased post-injury but no difference was detected compared to ethanol pretreatment. The increase in claudin-5 post-TBI was correlated with NFL but not with NSE or S100B.

CONCLUSIONS: Ethanol induces an effect on biomarker release in the bloodstream that is different from TBI not influenced by alcohol. This could be the basis of investigations into humans.

Original language	English
Journal	Burns & trauma
Volume	9
Pages (from-to)	tkab027
ISSN	2321-3868
DOIs	https://doi.org/10.1093/burnst/tkab027
Publication status	Published - 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/burnst/tkab027

Cite this

@article{960d77af7e174824804b42a09b053910,

title = "Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury",

abstract = "BACKGROUND: Blood-based biomarkers have proven to be a reliable measure of the severity and outcome of traumatic brain injury (TBI) in both murine models and patients. In particular, neuron-specific enolase (NSE), neurofilament light (NFL) and S100 beta (S100B) have been investigated in the clinical setting post-injury. Ethanol intoxication (EI) remains a significant comorbidity in TBI, with 30-40% of patients having a positive blood alcohol concentration post-TBI. The effect of ethanol on blood-based biomarkers for the prognosis and diagnosis of TBI remains unclear. In this study, we investigated the effect of EI on NSE, NFL and S100B and their correlation with blood-brain barrier integrity in a murine model of TBI.METHODS: We used ultra-sensitive single-molecule array technology and enzyme-linked immunosorbent assay methods to measure NFL, NSE, S100B and claudin-5 concentrations in plasma 3 hours post-TBI.RESULTS: We showed that NFL, NSE and S100B were increased at 3 hours post-TBI. Interestingly, ethanol blood concentrations showed an inverse correlation with NSE but not with NFL or S100B. Claudin-5 levels were increased post-injury but no difference was detected compared to ethanol pretreatment. The increase in claudin-5 post-TBI was correlated with NFL but not with NSE or S100B.CONCLUSIONS: Ethanol induces an effect on biomarker release in the bloodstream that is different from TBI not influenced by alcohol. This could be the basis of investigations into humans.",

author = "Zhenghui Li and Jin Zhang and Steffen Halbgebauer and Akila Chandrasekar and Rida Rehman and Albert Ludolph and Tobias Boeckers and Markus Huber-Lang and Markus Otto and Francesco Roselli and Heuvel, {Florian Olde}",

note = "{\textcopyright} The Author(s) 2021. Published by Oxford University Press.",

year = "2021",

doi = "10.1093/burnst/tkab027",

language = "English",

volume = "9",

pages = "tkab027",

journal = "Burns & trauma",

issn = "2321-3868",

}

TY - JOUR

T1 - Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury

AU - Li, Zhenghui

AU - Zhang, Jin

AU - Halbgebauer, Steffen

AU - Chandrasekar, Akila

AU - Rehman, Rida

AU - Ludolph, Albert

AU - Boeckers, Tobias

AU - Huber-Lang, Markus

AU - Otto, Markus

AU - Roselli, Francesco

AU - Heuvel, Florian Olde

PY - 2021

Y1 - 2021

N2 - BACKGROUND: Blood-based biomarkers have proven to be a reliable measure of the severity and outcome of traumatic brain injury (TBI) in both murine models and patients. In particular, neuron-specific enolase (NSE), neurofilament light (NFL) and S100 beta (S100B) have been investigated in the clinical setting post-injury. Ethanol intoxication (EI) remains a significant comorbidity in TBI, with 30-40% of patients having a positive blood alcohol concentration post-TBI. The effect of ethanol on blood-based biomarkers for the prognosis and diagnosis of TBI remains unclear. In this study, we investigated the effect of EI on NSE, NFL and S100B and their correlation with blood-brain barrier integrity in a murine model of TBI.METHODS: We used ultra-sensitive single-molecule array technology and enzyme-linked immunosorbent assay methods to measure NFL, NSE, S100B and claudin-5 concentrations in plasma 3 hours post-TBI.RESULTS: We showed that NFL, NSE and S100B were increased at 3 hours post-TBI. Interestingly, ethanol blood concentrations showed an inverse correlation with NSE but not with NFL or S100B. Claudin-5 levels were increased post-injury but no difference was detected compared to ethanol pretreatment. The increase in claudin-5 post-TBI was correlated with NFL but not with NSE or S100B.CONCLUSIONS: Ethanol induces an effect on biomarker release in the bloodstream that is different from TBI not influenced by alcohol. This could be the basis of investigations into humans.

AB - BACKGROUND: Blood-based biomarkers have proven to be a reliable measure of the severity and outcome of traumatic brain injury (TBI) in both murine models and patients. In particular, neuron-specific enolase (NSE), neurofilament light (NFL) and S100 beta (S100B) have been investigated in the clinical setting post-injury. Ethanol intoxication (EI) remains a significant comorbidity in TBI, with 30-40% of patients having a positive blood alcohol concentration post-TBI. The effect of ethanol on blood-based biomarkers for the prognosis and diagnosis of TBI remains unclear. In this study, we investigated the effect of EI on NSE, NFL and S100B and their correlation with blood-brain barrier integrity in a murine model of TBI.METHODS: We used ultra-sensitive single-molecule array technology and enzyme-linked immunosorbent assay methods to measure NFL, NSE, S100B and claudin-5 concentrations in plasma 3 hours post-TBI.RESULTS: We showed that NFL, NSE and S100B were increased at 3 hours post-TBI. Interestingly, ethanol blood concentrations showed an inverse correlation with NSE but not with NFL or S100B. Claudin-5 levels were increased post-injury but no difference was detected compared to ethanol pretreatment. The increase in claudin-5 post-TBI was correlated with NFL but not with NSE or S100B.CONCLUSIONS: Ethanol induces an effect on biomarker release in the bloodstream that is different from TBI not influenced by alcohol. This could be the basis of investigations into humans.

U2 - 10.1093/burnst/tkab027

DO - 10.1093/burnst/tkab027

M3 - Journal articles

C2 - 34604393

SN - 2321-3868

VL - 9

SP - tkab027

JO - Burns & trauma

JF - Burns & trauma

ER -

Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this