Different functional states of topoisomerase II can be discriminated by orthovanadate sensitivity in multi-drug resistant human leukemic cells

Fritz Boege; Frank Gieseler; Harald Biersack; Peter Meyer

doi:10.3109/10428199309148538

Different functional states of topoisomerase II can be discriminated by orthovanadate sensitivity in multi-drug resistant human leukemic cells

Fritz Boege^*, Frank Gieseler, Harald Biersack, Peter Meyer

^*Corresponding author for this work

Clinic of Internal Medicine I

University of Würzburg

2 Citations (Scopus)

Abstract

We have studied the functional properties of topoisomerase II (Topo II) in a subclone of the HL-60 cell line, which is highly resistant to cytotoxic Topo II inhibitors, but does not express p-glycoprotein. The cells contain the two forms of human topo II with Mr 170 and 180 kDa in equal proportions. Two different states of both forms of the enzymes can be separated by anion-exchange chromatography and functionally discriminated on the basis of orthovanadate sensitivity. The EC₅₀ of orthovanadate was 0.2 μM for the early eluting and 30 μM for the late eluting Topo II.

Original language	English
Journal	Leukemia and Lymphoma
Volume	9
Issue number	4-5
Pages (from-to)	381-383
Number of pages	3
ISSN	1042-8194
DOIs	https://doi.org/10.3109/10428199309148538
Publication status	Published - 1993

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title = "Different functional states of topoisomerase II can be discriminated by orthovanadate sensitivity in multi-drug resistant human leukemic cells",

abstract = "We have studied the functional properties of topoisomerase II (Topo II) in a subclone of the HL-60 cell line, which is highly resistant to cytotoxic Topo II inhibitors, but does not express p-glycoprotein. The cells contain the two forms of human topo II with Mr 170 and 180 kDa in equal proportions. Two different states of both forms of the enzymes can be separated by anion-exchange chromatography and functionally discriminated on the basis of orthovanadate sensitivity. The EC50 of orthovanadate was 0.2 μM for the early eluting and 30 μM for the late eluting Topo II.",

author = "Fritz Boege and Frank Gieseler and Harald Biersack and Peter Meyer",

note = "Funding Information: Acknowledgemenrs This work was in part supported by the Wilhelm-Sander Stiftung, grant 90.038.1. and the Deutsche Forschungsgemeinschaft, SFB 172, C9. The authors are much indebted to Prof. L. F. Liu for the gift of rabbit anti human toDoisomerase I1 antiserum. Excellent technical assistance bv M'ichaela Miiller and Michael Clark.",

year = "1993",

doi = "10.3109/10428199309148538",

language = "English",

volume = "9",

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journal = "Leukemia and Lymphoma",

issn = "1042-8194",

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T1 - Different functional states of topoisomerase II can be discriminated by orthovanadate sensitivity in multi-drug resistant human leukemic cells

AU - Boege, Fritz

AU - Gieseler, Frank

AU - Biersack, Harald

AU - Meyer, Peter

N1 - Funding Information: Acknowledgemenrs This work was in part supported by the Wilhelm-Sander Stiftung, grant 90.038.1. and the Deutsche Forschungsgemeinschaft, SFB 172, C9. The authors are much indebted to Prof. L. F. Liu for the gift of rabbit anti human toDoisomerase I1 antiserum. Excellent technical assistance bv M'ichaela Miiller and Michael Clark.

PY - 1993

Y1 - 1993

N2 - We have studied the functional properties of topoisomerase II (Topo II) in a subclone of the HL-60 cell line, which is highly resistant to cytotoxic Topo II inhibitors, but does not express p-glycoprotein. The cells contain the two forms of human topo II with Mr 170 and 180 kDa in equal proportions. Two different states of both forms of the enzymes can be separated by anion-exchange chromatography and functionally discriminated on the basis of orthovanadate sensitivity. The EC50 of orthovanadate was 0.2 μM for the early eluting and 30 μM for the late eluting Topo II.

AB - We have studied the functional properties of topoisomerase II (Topo II) in a subclone of the HL-60 cell line, which is highly resistant to cytotoxic Topo II inhibitors, but does not express p-glycoprotein. The cells contain the two forms of human topo II with Mr 170 and 180 kDa in equal proportions. Two different states of both forms of the enzymes can be separated by anion-exchange chromatography and functionally discriminated on the basis of orthovanadate sensitivity. The EC50 of orthovanadate was 0.2 μM for the early eluting and 30 μM for the late eluting Topo II.

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U2 - 10.3109/10428199309148538

DO - 10.3109/10428199309148538

M3 - Journal articles

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AN - SCOPUS:0027172014

SN - 1042-8194

VL - 9

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JO - Leukemia and Lymphoma

JF - Leukemia and Lymphoma

IS - 4-5

ER -

Different functional states of topoisomerase II can be discriminated by orthovanadate sensitivity in multi-drug resistant human leukemic cells

Abstract

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