Development of a noninvasive, laser-assisted experimental model of corneal endothelial cell loss

Annekatrin Holzhey, Svenja Sonntag, Johannes Rendenbach, Justus S. Ernesti, Vinodh Kakkassery, Salvatore Grisanti, Fred Reinholz, Sebastian Freidank, Alfred Vogel, Mahdy Ranjbar*

*Corresponding author for this work

Abstract

Nd:YAG lasers have been used to perform noninvasive intraocular surgery, such as capsulotomy for several decades now. The incisive effect relies on the optical breakdown at the laser focus. Acoustic shock waves and cavitation bubbles are generated, causing tissue rupture. Bubble sizes and pressure amplitudes vary with pulse energy and position of the focal point. In this study, enucleated porcine eyes were positioned in front of a commercially available Nd:YAG laser. Variable pulse energies as well as different positions of the focal spots posterior to the cornea were tested. Resulting lesions were evaluated by two-photon microscopy and histology to determine the best parameters for an exclusive detachment of corneal endothelial cells (CEC) with minimum collateral damage. The advantages of this method are the precise ablation of CEC, reduced collateral damage, and above all, the non-contact treatment.

Original languageEnglish
Article numbere60542
JournalJournal of Visualized Experiments
Volume2020
Issue number158
ISSN1940-087X
DOIs
Publication statusPublished - 04.2020

Research Areas and Centers

  • Research Area: Luebeck Integrated Oncology Network (LION)

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