TY - JOUR
T1 - Development of a noninvasive, laser-assisted experimental model of corneal endothelial cell loss
AU - Holzhey, Annekatrin
AU - Sonntag, Svenja
AU - Rendenbach, Johannes
AU - Ernesti, Justus S.
AU - Kakkassery, Vinodh
AU - Grisanti, Salvatore
AU - Reinholz, Fred
AU - Freidank, Sebastian
AU - Vogel, Alfred
AU - Ranjbar, Mahdy
PY - 2020/4
Y1 - 2020/4
N2 - Nd:YAG lasers have been used to perform noninvasive intraocular surgery, such as capsulotomy for several decades now. The incisive effect relies on the optical breakdown at the laser focus. Acoustic shock waves and cavitation bubbles are generated, causing tissue rupture. Bubble sizes and pressure amplitudes vary with pulse energy and position of the focal point. In this study, enucleated porcine eyes were positioned in front of a commercially available Nd:YAG laser. Variable pulse energies as well as different positions of the focal spots posterior to the cornea were tested. Resulting lesions were evaluated by two-photon microscopy and histology to determine the best parameters for an exclusive detachment of corneal endothelial cells (CEC) with minimum collateral damage. The advantages of this method are the precise ablation of CEC, reduced collateral damage, and above all, the non-contact treatment.
AB - Nd:YAG lasers have been used to perform noninvasive intraocular surgery, such as capsulotomy for several decades now. The incisive effect relies on the optical breakdown at the laser focus. Acoustic shock waves and cavitation bubbles are generated, causing tissue rupture. Bubble sizes and pressure amplitudes vary with pulse energy and position of the focal point. In this study, enucleated porcine eyes were positioned in front of a commercially available Nd:YAG laser. Variable pulse energies as well as different positions of the focal spots posterior to the cornea were tested. Resulting lesions were evaluated by two-photon microscopy and histology to determine the best parameters for an exclusive detachment of corneal endothelial cells (CEC) with minimum collateral damage. The advantages of this method are the precise ablation of CEC, reduced collateral damage, and above all, the non-contact treatment.
UR - http://www.scopus.com/inward/record.url?scp=85084695823&partnerID=8YFLogxK
U2 - 10.3791/60542
DO - 10.3791/60542
M3 - Journal articles
C2 - 32391812
AN - SCOPUS:85084695823
SN - 1940-087X
VL - 2020
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 158
M1 - e60542
ER -
