Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue

Sebastian Siebelmann; Uta Gehlsen; Gereon Hüttmann; Norbert Koop; Torsten Bölke; Andreas Gebert; Michael E. Stern; Jerry Y. Niederkorn; Philipp Steven

doi:10.1371/journal.pone.0082355

Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue

Sebastian Siebelmann, Uta Gehlsen, Gereon Hüttmann, Norbert Koop, Torsten Bölke, Andreas Gebert, Michael E. Stern, Jerry Y. Niederkorn, Philipp Steven

41 Citations (Scopus)

Abstract

Purpose: Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model. Methods: Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy. Results: Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 μm/min. Conclusions: CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration. Copyright:

Original language	English
Article number	e82355
Journal	PLoS ONE
Volume	8
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0082355
Publication status	Published - 20.12.2013

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Academic Focus: Biomedical Engineering

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10.1371/journal.pone.0082355

Cite this

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title = "Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue",

abstract = "Purpose: Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model. Methods: Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy. Results: Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 μm/min. Conclusions: CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration. Copyright:",

author = "Sebastian Siebelmann and Uta Gehlsen and Gereon H{\"u}ttmann and Norbert Koop and Torsten B{\"o}lke and Andreas Gebert and Stern, \{Michael E.\} and Niederkorn, \{Jerry Y.\} and Philipp Steven",

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AU - Siebelmann, Sebastian

AU - Gehlsen, Uta

AU - Hüttmann, Gereon

AU - Koop, Norbert

AU - Bölke, Torsten

AU - Gebert, Andreas

AU - Stern, Michael E.

AU - Niederkorn, Jerry Y.

AU - Steven, Philipp

PY - 2013/12/20

Y1 - 2013/12/20

N2 - Purpose: Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model. Methods: Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy. Results: Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 μm/min. Conclusions: CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration. Copyright:

AB - Purpose: Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model. Methods: Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy. Results: Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 μm/min. Conclusions: CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration. Copyright:

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Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue

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