Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue

Sebastian Siebelmann, Uta Gehlsen, Gereon Hüttmann, Norbert Koop, Torsten Bölke, Andreas Gebert, Michael E. Stern, Jerry Y. Niederkorn, Philipp Steven

26 Citations (Scopus)


Purpose: Conjunctiva-associated lymphoid tissue (CALT) is thought to play a key role in initiating ocular surface related immune responses. This study was planned to get first profound insights into the function of CALT related to development, cellular dynamics and morphological alteration using a novel mouse model. Methods: Expression and morphology of CALT were investigated using BALB/c mice kept under different housing conditions, after topical antigen-stimulation and following lymphadenectomy and splenectomy. Particles and bacteria were applied topically to study antigen-transport. Intravital visualization was performed using two-photon microscopy. Results: Postnatal development and ultrastructure of CALT in the mouse is similar to humans. Topical antigen-challenge significantly alters CALT expression. Bacterial translocation is demonstrated via lymphoepithelium whereas cellular velocities within follicles were approximately 8 μm/min. Conclusions: CALT in the mouse is an immunological interface of the ocular surface, featuring dynamic processes such as morphological plasticity, particle/bacteria transport and cellular migration. Copyright:

Original languageEnglish
Article numbere82355
JournalPLoS ONE
Issue number12
Publication statusPublished - 20.12.2013

Research Areas and Centers

  • Academic Focus: Biomedical Engineering


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