Transforming growth factors β (TGFβs) show pleotrophic functions in vitro and in vivo. Biological effects depend on the cell type, the TGFβ isoform, and the availability of active TGFβ. Bioassays for TGFβ have not proven to be reliable tools in the measurement of TGFβ in human blood samples. Previously described sandwich ELISAs for measuring TGFβ are limited to single TGFβ isoforms and have not been evaluated for use in human sera or plasma. We describe a simple procedure to quantitate not only TGFβ1 but also TGFβ2 in human blood samples using commercially available antibodies. The sensitivity of the TGFβ2 ELISA was 11 pg/ml. There was no crossreactivity between the TGFβ1 and TGFβ3 isoforms. High concentrations of TGFβ1 and TGFβ3 in spiked samples did not interfere with TGFβ2 determination. TGFβ2 recovery was highest in EDTA plasma (> 88%), and the intra-and interassay variance was 6% and 8.5% respectively. Applying the ELISA to human plasma specimens a significant percentage of TGFβ2 (3.7 ± 0.8 ng/ml) was found (about 50% of the TGFβ1 content).
Research Areas and Centers
- Academic Focus: Center for Infection and Inflammation Research (ZIEL)