TY - JOUR
T1 - Determination of transforming growth factor β2 in human blood samples by ELISA
AU - Szymkowiak, Christof H.
AU - Mons, Ingrid
AU - Gross, Wolfgang L.
AU - Kekow, Jörn
N1 - Funding Information:
This work was supported by grants from the Bundesministerium fiir Forschung und Technolo-gie (autoimmunity grant, 01 KD 89030), Bonn, and from the Deutsche Forschungsgemeinschaft (grant GR 607/7-l), Bonn. Funds were also provided by the Verein zur Forderung der Er-forschung und Beklmpfung rheumatischer Erkrankungen e.V., Bad Bramstedt, Germany.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1995/8/18
Y1 - 1995/8/18
N2 - Transforming growth factors β (TGFβs) show pleotrophic functions in vitro and in vivo. Biological effects depend on the cell type, the TGFβ isoform, and the availability of active TGFβ. Bioassays for TGFβ have not proven to be reliable tools in the measurement of TGFβ in human blood samples. Previously described sandwich ELISAs for measuring TGFβ are limited to single TGFβ isoforms and have not been evaluated for use in human sera or plasma. We describe a simple procedure to quantitate not only TGFβ1 but also TGFβ2 in human blood samples using commercially available antibodies. The sensitivity of the TGFβ2 ELISA was 11 pg/ml. There was no crossreactivity between the TGFβ1 and TGFβ3 isoforms. High concentrations of TGFβ1 and TGFβ3 in spiked samples did not interfere with TGFβ2 determination. TGFβ2 recovery was highest in EDTA plasma (> 88%), and the intra-and interassay variance was 6% and 8.5% respectively. Applying the ELISA to human plasma specimens a significant percentage of TGFβ2 (3.7 ± 0.8 ng/ml) was found (about 50% of the TGFβ1 content).
AB - Transforming growth factors β (TGFβs) show pleotrophic functions in vitro and in vivo. Biological effects depend on the cell type, the TGFβ isoform, and the availability of active TGFβ. Bioassays for TGFβ have not proven to be reliable tools in the measurement of TGFβ in human blood samples. Previously described sandwich ELISAs for measuring TGFβ are limited to single TGFβ isoforms and have not been evaluated for use in human sera or plasma. We describe a simple procedure to quantitate not only TGFβ1 but also TGFβ2 in human blood samples using commercially available antibodies. The sensitivity of the TGFβ2 ELISA was 11 pg/ml. There was no crossreactivity between the TGFβ1 and TGFβ3 isoforms. High concentrations of TGFβ1 and TGFβ3 in spiked samples did not interfere with TGFβ2 determination. TGFβ2 recovery was highest in EDTA plasma (> 88%), and the intra-and interassay variance was 6% and 8.5% respectively. Applying the ELISA to human plasma specimens a significant percentage of TGFβ2 (3.7 ± 0.8 ng/ml) was found (about 50% of the TGFβ1 content).
UR - http://www.scopus.com/inward/record.url?scp=0029056184&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(95)00098-U
DO - 10.1016/0022-1759(95)00098-U
M3 - Journal articles
C2 - 7658028
AN - SCOPUS:0029056184
SN - 0022-1759
VL - 184
SP - 263
EP - 271
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -
