Abstract
A method is presented to determine the RNA content in isolated chromatin. An increased ellipticity at 260 nm-290 nm in the circular dichroism spectrum of chromatin is due to RNA released together with chromatin from the cell nuclei. Mixtures of chicken erythrocyte chromatin and pure 5s-RNA were used to reconstruct such spectra. From the obtained data an RNA content higher than 3-5% in samples of isolated chromatin can be determined.
| Original language | English |
|---|---|
| Journal | Molecular biology reports |
| Volume | 13 |
| Issue number | 1 |
| Pages (from-to) | 15-9 |
| Number of pages | 5 |
| ISSN | 0301-4851 |
| Publication status | Published - 1988 |
