Detection of cell membrane-bound CD46 using flow cytometry

Martin Kolev; Claudia Kemper

doi:10.1007/978-1-62703-724-2_27

Detection of cell membrane-bound CD46 using flow cytometry

Martin Kolev, Claudia Kemper

Institute of Systemic Inflammation Research

King's College London

4 Citations (Scopus)

Abstract

CD46 is an important regulator of the complement system by preventing unwanted deposition of the complement activation products and opsonins C3b/C4b onto self-tissue. Recently, intracellular signals mediated by CD46 activation on several distinct human cell types have demonstrated that CD46 also plays decisive roles in immuneregulation. The growing recognition of CD46 as key regulator in several vital biological processes, led to increased demand in sensitive methods for monitoring CD46 expression and changes thereof on cells and in tissues. Here we describe a method, which allows for studying CD46 expression on the surface of cells using specific antibodies in combination with fluorescence-activated cell sorting (FACS) analysis.

Original language	English
Title of host publication	The Complement System: Methods and Protocols
Number of pages	11
Publisher	Humana Press Inc.
Publication date	2014
Pages	329-339
ISBN (Print)	9781627037235
DOIs	https://doi.org/10.1007/978-1-62703-724-2_27
Publication status	Published - 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1007/978-1-62703-724-2_27

Cite this

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AB - CD46 is an important regulator of the complement system by preventing unwanted deposition of the complement activation products and opsonins C3b/C4b onto self-tissue. Recently, intracellular signals mediated by CD46 activation on several distinct human cell types have demonstrated that CD46 also plays decisive roles in immuneregulation. The growing recognition of CD46 as key regulator in several vital biological processes, led to increased demand in sensitive methods for monitoring CD46 expression and changes thereof on cells and in tissues. Here we describe a method, which allows for studying CD46 expression on the surface of cells using specific antibodies in combination with fluorescence-activated cell sorting (FACS) analysis.

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BT - The Complement System: Methods and Protocols

PB - Humana Press Inc.

ER -

Detection of cell membrane-bound CD46 using flow cytometry

Abstract

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