Detection of cell membrane-bound CD46 using flow cytometry

Martin Kolev, Claudia Kemper

1 Citation (Scopus)


CD46 is an important regulator of the complement system by preventing unwanted deposition of the complement activation products and opsonins C3b/C4b onto self-tissue. Recently, intracellular signals mediated by CD46 activation on several distinct human cell types have demonstrated that CD46 also plays decisive roles in immuneregulation. The growing recognition of CD46 as key regulator in several vital biological processes, led to increased demand in sensitive methods for monitoring CD46 expression and changes thereof on cells and in tissues. Here we describe a method, which allows for studying CD46 expression on the surface of cells using specific antibodies in combination with fluorescence-activated cell sorting (FACS) analysis.

Original languageEnglish
Title of host publicationThe Complement System: Methods and Protocols
Number of pages11
PublisherHumana Press Inc.
Publication date2014
ISBN (Print)9781627037235
Publication statusPublished - 2014

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)


Dive into the research topics of 'Detection of cell membrane-bound CD46 using flow cytometry'. Together they form a unique fingerprint.

Cite this