Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system

Marjeta Hofko; Alexander Mischnik; Martin Kaase; Stefan Zimmermann; Alexander H. Dalpke

doi:10.1128/JCM.00373-14

Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system

Marjeta Hofko, Alexander Mischnik, Martin Kaase, Stefan Zimmermann, Alexander H. Dalpke^*

^*Corresponding author for this work

29 Citations (Scopus)

Abstract

We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	52
Issue number	5
Pages (from-to)	1701-1704
Number of pages	4
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.00373-14
Publication status	Published - 01.01.2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1128/JCM.00373-14

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abstract = "We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.",

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AU - Kaase, Martin

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AU - Dalpke, Alexander H.

PY - 2014/1/1

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Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system

Abstract

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