Derivation and characterization of chondrocytes from embryonic stem cells in vitro.

Jan Kramer; Gunnar Hargus; Jürgen Rohwedel

doi:10.1385/1-59745-036-7:171

Derivation and characterization of chondrocytes from embryonic stem cells in vitro.

Jan Kramer^*, Gunnar Hargus, Jürgen Rohwedel

^*Corresponding author for this work

2 Citations (Scopus)

Abstract

The model system of embryonic stem (ES) cell differentiation in vitro via cellular aggregates (embryoid bodies, EBs) can be used to analyze cell differentiation from a pluripotent stem cell via progenitor cells up to terminally differentiated cell types. ES cells are known to be pluripotent; they have the capacity to differentiate into any cell lineage of the three germ layers. Using various ES cell lines, we characterized chondrogenic and osteogenic differentiation in EBs by histochemical staining, immunostaining, mRNA-in situ hybridization, and reverse transcriptase polymerase chain reaction analysis. Here, we describe in detail our established protocols to analyze chondrogenic differentiation of ES cells. We summarize different ways to modulate ES cell-derived chondrogenic differentiation.

Original language	English
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	330
Pages (from-to)	171-190
Number of pages	20
ISSN	1064-3745
DOIs	https://doi.org/10.1385/1-59745-036-7:171
Publication status	Published - 24.08.2006

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Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1385/1-59745-036-7:171

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title = "Derivation and characterization of chondrocytes from embryonic stem cells in vitro.",

abstract = "The model system of embryonic stem (ES) cell differentiation in vitro via cellular aggregates (embryoid bodies, EBs) can be used to analyze cell differentiation from a pluripotent stem cell via progenitor cells up to terminally differentiated cell types. ES cells are known to be pluripotent; they have the capacity to differentiate into any cell lineage of the three germ layers. Using various ES cell lines, we characterized chondrogenic and osteogenic differentiation in EBs by histochemical staining, immunostaining, mRNA-in situ hybridization, and reverse transcriptase polymerase chain reaction analysis. Here, we describe in detail our established protocols to analyze chondrogenic differentiation of ES cells. We summarize different ways to modulate ES cell-derived chondrogenic differentiation.",

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AU - Hargus, Gunnar

AU - Rohwedel, Jürgen

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N2 - The model system of embryonic stem (ES) cell differentiation in vitro via cellular aggregates (embryoid bodies, EBs) can be used to analyze cell differentiation from a pluripotent stem cell via progenitor cells up to terminally differentiated cell types. ES cells are known to be pluripotent; they have the capacity to differentiate into any cell lineage of the three germ layers. Using various ES cell lines, we characterized chondrogenic and osteogenic differentiation in EBs by histochemical staining, immunostaining, mRNA-in situ hybridization, and reverse transcriptase polymerase chain reaction analysis. Here, we describe in detail our established protocols to analyze chondrogenic differentiation of ES cells. We summarize different ways to modulate ES cell-derived chondrogenic differentiation.

AB - The model system of embryonic stem (ES) cell differentiation in vitro via cellular aggregates (embryoid bodies, EBs) can be used to analyze cell differentiation from a pluripotent stem cell via progenitor cells up to terminally differentiated cell types. ES cells are known to be pluripotent; they have the capacity to differentiate into any cell lineage of the three germ layers. Using various ES cell lines, we characterized chondrogenic and osteogenic differentiation in EBs by histochemical staining, immunostaining, mRNA-in situ hybridization, and reverse transcriptase polymerase chain reaction analysis. Here, we describe in detail our established protocols to analyze chondrogenic differentiation of ES cells. We summarize different ways to modulate ES cell-derived chondrogenic differentiation.

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JO - Methods in molecular biology (Clifton, N.J.)

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Derivation and characterization of chondrocytes from embryonic stem cells in vitro.

Abstract

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