Abstract
Dendritic cells (DC) are important in differential T-cell priming. Little is known about the local priming by DC in the microenvironment of different lymph nodes and about the fate of the imprinted T cells. Therefore, freshly isolated rat DC from mesenteric lymph nodes (mLN) and axillary lymph nodes (axLN) were phenotyped and cultured with blood T cells in the presence of the superantigen Mycoplasma arthritidis mitogen (MAM). The phenotype, proliferation and apoptosis of the primed T cells were analysed. Our data show that a common DC population exists in both mLN and axLN. In addition, region-specific DC with an organotypical marker expression imprinted by the drained area were found. Coculture of T cells with DC from mLN or axLN resulted in a distinct shift in the CD4 and CD8 expression of T cells and their phenotype. Furthermore, when these differentially primed mLN and axLN T cells were injected into recipients, mLN-primed T cells survived longer in other lymphoid organs. The results show that the region-specific DC have a unique phenotype and an impact on the ratio of CD4:CD8 T cells during an immune response in vivo.
Original language | English |
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Journal | Immunology |
Volume | 123 |
Issue number | 4 |
Pages (from-to) | 480-490 |
Number of pages | 11 |
ISSN | 0019-2805 |
DOIs | |
Publication status | Published - 04.2008 |
Research Areas and Centers
- Academic Focus: Center for Infection and Inflammation Research (ZIEL)