Dedicated Chamber for Multimodal in Vivo Imaging of Adult Zebrafish

Steven Seeger; Milan Zvolský; Salim Melikov; Maja Frerkes; Magdalena Rafecas

doi:10.1089/zeb.2021.0066

Dedicated Chamber for Multimodal in Vivo Imaging of Adult Zebrafish

Steven Seeger, Milan Zvolský, Salim Melikov, Maja Frerkes, Magdalena Rafecas

Institute of Medical Engineering

Abstract

In vivo imaging of adult zebrafish is challenging, particularly for dynamic or long acquisitions when using, for example, positron emission tomography, single photon emission computed tomography, computed tomography (CT), or magnetic resonance imaging (MRI). An aqueous environment is indispensable to ensure animal welfare, but commercial small-Animal imaging chambers do not provide such conditions, as they are designed for rodents. In this study we present a dedicated flow-Through chamber that includes fish immobilization and allows for the continuous supply of fresh water and anesthetics, as well as the removal of excretions. Both flow simulations and experiments, as well as first scans with MRI and CT, support the suitability of the chamber.

Original language	English
Journal	Zebrafish
Volume	19
Issue number	2
Pages (from-to)	67-70
Number of pages	4
DOIs	https://doi.org/10.1089/zeb.2021.0066
Publication status	Published - 01.2022

Research Areas and Centers

Academic Focus: Biomedical Engineering

DFG Research Classification Scheme

2.22-32 Medical Physics, Biomedical Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/zeb.2021.0066

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AB - In vivo imaging of adult zebrafish is challenging, particularly for dynamic or long acquisitions when using, for example, positron emission tomography, single photon emission computed tomography, computed tomography (CT), or magnetic resonance imaging (MRI). An aqueous environment is indispensable to ensure animal welfare, but commercial small-Animal imaging chambers do not provide such conditions, as they are designed for rodents. In this study we present a dedicated flow-Through chamber that includes fish immobilization and allows for the continuous supply of fresh water and anesthetics, as well as the removal of excretions. Both flow simulations and experiments, as well as first scans with MRI and CT, support the suitability of the chamber.

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Dedicated Chamber for Multimodal in Vivo Imaging of Adult Zebrafish

Abstract

Research Areas and Centers

DFG Research Classification Scheme

UN SDGs

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