Decreased migration of myeloid dendritic cells through increased levels of C-reactive protein

Henning Frenzel; Ralph Pries; Carsten P. Brocks; Wolfram J. Jabs; Nadine Wittkopf; Barbara Wollenberg

Decreased migration of myeloid dendritic cells through increased levels of C-reactive protein

Henning Frenzel, Ralph Pries, Carsten P. Brocks, Wolfram J. Jabs, Nadine Wittkopf, Barbara Wollenberg^*

^*Corresponding author for this work

Abstract

Background: In head and neck squamous cell carcinoma (HNSCC), a variety of immunomodulatory mediators contribute to strongly impaired immune functions. The secretion of C-reactive protein (CRP) by HNSCC cells and its influence on human myeloid dendritic cells (MDC) was investigated. Materials and Methods: The CRP levels were analyzed using photometric methods and real-time PCR. The MDC were isolated from peripheral blood by 'magnetic bead separation' and incubated with different CRP concentrations. The CRP isoforms were analyzed by native PAGE (polyacrylamide gel electrophoresis). The cells were analyzed using migration assays and flow cytometry. Results: HNSCC cell lines were able to autonomously express C-reactive protein. Pentameric CRP triggered the down-regulation of chemokine receptor CCR5 and led to a decreased migration of human MDC. Conclusion: CRP appeared to be a modulator of the migration activity of human MDC. The functional modulation of immune cells represents a crucial immune escape mechanism of human carcinomas.

Original language	English
Journal	Anticancer Research
Volume	27
Issue number	6 B
Pages (from-to)	4111-4115
Number of pages	5
ISSN	0250-7005
Publication status	Published - 11.2007

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@article{28b57cb4102641c7921e48b8aa3b3211,

title = "Decreased migration of myeloid dendritic cells through increased levels of C-reactive protein",

abstract = "Background: In head and neck squamous cell carcinoma (HNSCC), a variety of immunomodulatory mediators contribute to strongly impaired immune functions. The secretion of C-reactive protein (CRP) by HNSCC cells and its influence on human myeloid dendritic cells (MDC) was investigated. Materials and Methods: The CRP levels were analyzed using photometric methods and real-time PCR. The MDC were isolated from peripheral blood by 'magnetic bead separation' and incubated with different CRP concentrations. The CRP isoforms were analyzed by native PAGE (polyacrylamide gel electrophoresis). The cells were analyzed using migration assays and flow cytometry. Results: HNSCC cell lines were able to autonomously express C-reactive protein. Pentameric CRP triggered the down-regulation of chemokine receptor CCR5 and led to a decreased migration of human MDC. Conclusion: CRP appeared to be a modulator of the migration activity of human MDC. The functional modulation of immune cells represents a crucial immune escape mechanism of human carcinomas.",

author = "Henning Frenzel and Ralph Pries and Brocks, {Carsten P.} and Jabs, {Wolfram J.} and Nadine Wittkopf and Barbara Wollenberg",

year = "2007",

month = nov,

language = "English",

volume = "27",

pages = "4111--4115",

journal = "Anticancer Research",

issn = "0250-7005",

number = "6 B",

}

TY - JOUR

T1 - Decreased migration of myeloid dendritic cells through increased levels of C-reactive protein

AU - Frenzel, Henning

AU - Pries, Ralph

AU - Brocks, Carsten P.

AU - Jabs, Wolfram J.

AU - Wittkopf, Nadine

AU - Wollenberg, Barbara

PY - 2007/11

Y1 - 2007/11

N2 - Background: In head and neck squamous cell carcinoma (HNSCC), a variety of immunomodulatory mediators contribute to strongly impaired immune functions. The secretion of C-reactive protein (CRP) by HNSCC cells and its influence on human myeloid dendritic cells (MDC) was investigated. Materials and Methods: The CRP levels were analyzed using photometric methods and real-time PCR. The MDC were isolated from peripheral blood by 'magnetic bead separation' and incubated with different CRP concentrations. The CRP isoforms were analyzed by native PAGE (polyacrylamide gel electrophoresis). The cells were analyzed using migration assays and flow cytometry. Results: HNSCC cell lines were able to autonomously express C-reactive protein. Pentameric CRP triggered the down-regulation of chemokine receptor CCR5 and led to a decreased migration of human MDC. Conclusion: CRP appeared to be a modulator of the migration activity of human MDC. The functional modulation of immune cells represents a crucial immune escape mechanism of human carcinomas.

AB - Background: In head and neck squamous cell carcinoma (HNSCC), a variety of immunomodulatory mediators contribute to strongly impaired immune functions. The secretion of C-reactive protein (CRP) by HNSCC cells and its influence on human myeloid dendritic cells (MDC) was investigated. Materials and Methods: The CRP levels were analyzed using photometric methods and real-time PCR. The MDC were isolated from peripheral blood by 'magnetic bead separation' and incubated with different CRP concentrations. The CRP isoforms were analyzed by native PAGE (polyacrylamide gel electrophoresis). The cells were analyzed using migration assays and flow cytometry. Results: HNSCC cell lines were able to autonomously express C-reactive protein. Pentameric CRP triggered the down-regulation of chemokine receptor CCR5 and led to a decreased migration of human MDC. Conclusion: CRP appeared to be a modulator of the migration activity of human MDC. The functional modulation of immune cells represents a crucial immune escape mechanism of human carcinomas.

UR - http://www.scopus.com/inward/record.url?scp=37549036603&partnerID=8YFLogxK

M3 - Journal articles

C2 - 18225580

AN - SCOPUS:37549036603

SN - 0250-7005

VL - 27

SP - 4111

EP - 4115

JO - Anticancer Research

JF - Anticancer Research

IS - 6 B

ER -

Decreased migration of myeloid dendritic cells through increased levels of C-reactive protein

Abstract

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