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Cytochemical localization of guanylate cyclase in photoreceptor cells of the mouse

Ulrich Schraermeyer*, Peter Esser, Salvatore Grisanti, Michael Rack, Klaus Heimann

*Corresponding author for this work

Abstract

Background: Light-stimulated excitation causes a decrease of the cGMP concentration in vertebrate photoreceptor cells. The cGMP content is restored by the catalytic action of a guanylate cyclase (EC 4.6.1.2). Methods: The spatial distribution of guanylate cyclase was determined cytochemically in rod visual cells of the mouse. Results: In retinal tissue of the mouse guanylate cyclase was found throughout the photoreceptor cells, in the outer and the inner segments, and was especially prominent in the cilia and in elongations of cilia extending into the outer segments. A reaction product of adenylate cyclase (EC 4.6.1.1) could not be demonstrated in vertebrate rod outer segments. Conclusion: The relatively high amount of guanylate cyclase in the inner segments and the cilia may contribute - at least in part - to the actual concentration and the time course of concentration changes of the cGMP concentration in rod outer segments.

Original languageEnglish
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume235
Issue number3
Pages (from-to)176-179
Number of pages4
ISSN0721-832X
DOIs
Publication statusPublished - 1997

Funding

Acknowledgments We are very grateful to Marianne Dohms for excellent electron microscopy and photographic assistance and to Helga Gaube ]:or help with the manuscript. This study was supported by the Deutsche Forschungsgemeinschaft He 840/5-1; Es 82/5-1 and by the Retinovit Foundation.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

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