TY - JOUR
T1 - Cytochemical localization of guanylate cyclase in photoreceptor cells of the mouse
AU - Schraermeyer, Ulrich
AU - Esser, Peter
AU - Grisanti, Salvatore
AU - Rack, Michael
AU - Heimann, Klaus
N1 - Funding Information:
Acknowledgments We are very grateful to Marianne Dohms for excellent electron microscopy and photographic assistance and to Helga Gaube ]:or help with the manuscript. This study was supported by the Deutsche Forschungsgemeinschaft He 840/5-1; Es 82/5-1 and by the Retinovit Foundation.
PY - 1997
Y1 - 1997
N2 - Background: Light-stimulated excitation causes a decrease of the cGMP concentration in vertebrate photoreceptor cells. The cGMP content is restored by the catalytic action of a guanylate cyclase (EC 4.6.1.2). Methods: The spatial distribution of guanylate cyclase was determined cytochemically in rod visual cells of the mouse. Results: In retinal tissue of the mouse guanylate cyclase was found throughout the photoreceptor cells, in the outer and the inner segments, and was especially prominent in the cilia and in elongations of cilia extending into the outer segments. A reaction product of adenylate cyclase (EC 4.6.1.1) could not be demonstrated in vertebrate rod outer segments. Conclusion: The relatively high amount of guanylate cyclase in the inner segments and the cilia may contribute - at least in part - to the actual concentration and the time course of concentration changes of the cGMP concentration in rod outer segments.
AB - Background: Light-stimulated excitation causes a decrease of the cGMP concentration in vertebrate photoreceptor cells. The cGMP content is restored by the catalytic action of a guanylate cyclase (EC 4.6.1.2). Methods: The spatial distribution of guanylate cyclase was determined cytochemically in rod visual cells of the mouse. Results: In retinal tissue of the mouse guanylate cyclase was found throughout the photoreceptor cells, in the outer and the inner segments, and was especially prominent in the cilia and in elongations of cilia extending into the outer segments. A reaction product of adenylate cyclase (EC 4.6.1.1) could not be demonstrated in vertebrate rod outer segments. Conclusion: The relatively high amount of guanylate cyclase in the inner segments and the cilia may contribute - at least in part - to the actual concentration and the time course of concentration changes of the cGMP concentration in rod outer segments.
UR - http://www.scopus.com/inward/record.url?scp=0030934610&partnerID=8YFLogxK
U2 - 10.1007/BF00941725
DO - 10.1007/BF00941725
M3 - Journal articles
C2 - 9085113
AN - SCOPUS:0030934610
SN - 0721-832X
VL - 235
SP - 176
EP - 179
JO - Graefe's Archive for Clinical and Experimental Ophthalmology
JF - Graefe's Archive for Clinical and Experimental Ophthalmology
IS - 3
ER -
