Cuprizone [bis(cyclohexylidenehydrazide)] is selectively toxic for mature oligodendrocytes

Karelle Bénardais, Alexandra Kotsiari, Jelena Škuljec, Paraskevi N. Koutsoudaki, Viktoria Gudi, Vikramjeet Singh, Franca Vulinović, Thomas Skripuletz, Martin Stangel*

*Corresponding author for this work
39 Citations (Scopus)


Cuprizone [bis(cyclohexylidenehydrazide)]-induced toxic demyelination is an experimental animal model commonly used to study de- and remyelination in the central nervous system. In this model, mice are fed with the copper chelator cuprizone which leads to oligodendrocyte death with subsequent demyelination. The underlying mechanisms of cuprizone-induced oligodendrocyte death are still unknown, and appropriate in vitro investigations to study these mechanisms are not available. Thus, we studied cuprizone effects on rat primary glial cell cultures and on the neuroblastoma cell line SH-SY5Y. Treatment of cells with different concentrations of cuprizone failed to show effects on the proliferation and survival of SH-SY5Y cells, microglia, astrocytes, and oligodendrocyte precursor cells (OPC). In contrast, differentiated mature oligodendrocytes (OL) were found to be significantly affected by cuprizone treatment. This was accompanied by a reduced mitochondrial potential in cuprizone-treated OL. These results demonstrate that the main toxic target for cuprizone is mature OL, whilst other glial cells including OPC are not or only marginally affected. This explains the selective demyelination induced by cuprizone in vivo.

Original languageEnglish
JournalNeurotoxicity Research
Issue number2
Pages (from-to)244-250
Number of pages7
Publication statusPublished - 01.08.2013


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