Cumulative pregnancy rate after ICSI with cryopreserved testicular tissue in non-obstructive azoospermia

Konstantinos Dafopoulos, Georg Griesinger, Askan Schultze-Mosgau, Yasser Orief, Beate Schöpper, Nikos Nikolettos, Klaus Diedrich, Safaa Al-Hasani*

*Corresponding author for this work
24 Citations (Scopus)


The aim of the present study was to describe a simplified and inexpensive method of testicular tissue freezing to assess the cumulative clinical pregnancy rate (CPR) by this technique, and to provide useful information for counselling couple with non-obstructive azoospermia. One hundred and sixty-five couples with non-obstructive azoospermic males pursuing assisted conception, from December 1995 to December 2002, were included. In all cases, the testicular tissue retrieved by open multiple-biopsy (both sides, by testicular sperm extraction) was frozen using a simple liquid nitrogen vapour freezing technique and was stored in liquid nitrogen thereafter. Only mature spermatozoa were used for intracytoplasmic sperm injection (ICSI) after thawing. Expected CPR were calculated using the Kaplan-Meier survival analysis. A total of 281 cycles were performed resulting in 53 clinical pregnancies. Crude and expected CPR (95% confidence intervals) after three cycles were 32.1 (25.7-40.1) and 55.7% (37.0-74.4) respectively. In conclusion, this simplified method for freezing testicular tissue resulted in a satisfactory outcome after ICSI in cases of non-obstructive azoospermia.

Original languageEnglish
Article number1524
JournalReproductive BioMedicine Online
Issue number4
Pages (from-to)461-466
Number of pages6
Publication statusPublished - 04.2005


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