Crystals of intact elongation factor Tu from Thermus thermophilus diffracting to 1.45 Å resolution

L. S. Reshetnikova; N. K. Schirmer; C. O.A. Reiser; H. Berchtold; R. Storm; R. Hilgenfeld; M. Sprinzl

doi:10.1016/0022-0248(92)90270-S

Crystals of intact elongation factor Tu from Thermus thermophilus diffracting to 1.45 Å resolution

L. S. Reshetnikova, N. K. Schirmer, C. O.A. Reiser, H. Berchtold, R. Storm, R. Hilgenfeld, M. Sprinzl^*

^*Corresponding author for this work

Institute of Biochemistry

2 Citations (Scopus)

Abstract

Intact elongation factor Tu from Thermus thermophilus HB8 was crystallized in the presence of guanosine-5'-diphosphate (GDP), guanosine-5'-(β,γ-imido)triphosphate (GPPNP), guanosine-5'-(β,γ-methylene)triphosphate (GPPCP), p³-1-(2-nitro)phenylethylguanosine- 5'-triphosphate (caged GTP) and elongation factor Ts. An attempt to crystallize a ternary complex of EF-Tu·GPPNP with Phe-tRNA^Phe was made. The crystals of EF-Tu obtained with GPPNP or GPPCP, respectively, were suitable for X-ray diffraction analysis at very high resolution.

Original language	English
Journal	Journal of Crystal Growth
Volume	122
Issue number	1-4
Pages (from-to)	360-365
Number of pages	6
ISSN	0022-0248
DOIs	https://doi.org/10.1016/0022-0248(92)90270-S
Publication status	Published - 02.08.1992

Funding

We thank A. LOw for HPLC analysis. This work was supported by the Deutsche Forschungs-gemeinschaft, SFB 213.

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0022-0248(92)90270-S

Cite this

@article{a11d7eab9aa6430d801416cace32b5d5,

title = "Crystals of intact elongation factor Tu from Thermus thermophilus diffracting to 1.45 {\AA} resolution",

abstract = "Intact elongation factor Tu from Thermus thermophilus HB8 was crystallized in the presence of guanosine-5'-diphosphate (GDP), guanosine-5'-(β,γ-imido)triphosphate (GPPNP), guanosine-5'-(β,γ-methylene)triphosphate (GPPCP), p3-1-(2-nitro)phenylethylguanosine- 5'-triphosphate (caged GTP) and elongation factor Ts. An attempt to crystallize a ternary complex of EF-Tu·GPPNP with Phe-tRNAPhe was made. The crystals of EF-Tu obtained with GPPNP or GPPCP, respectively, were suitable for X-ray diffraction analysis at very high resolution.",

author = "Reshetnikova, \{L. S.\} and Schirmer, \{N. K.\} and Reiser, \{C. O.A.\} and H. Berchtold and R. Storm and R. Hilgenfeld and M. Sprinzl",

note = "Funding Information: We thank A. LOw for HPLC analysis. This work was supported by the Deutsche Forschungs-gemeinschaft, SFB 213. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1992",

month = aug,

day = "2",

doi = "10.1016/0022-0248(92)90270-S",

language = "English",

volume = "122",

pages = "360--365",

journal = "Journal of Crystal Growth",

issn = "0022-0248",

publisher = "Elsevier B.V.",

number = "1-4",

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TY - JOUR

T1 - Crystals of intact elongation factor Tu from Thermus thermophilus diffracting to 1.45 Å resolution

AU - Reshetnikova, L. S.

AU - Schirmer, N. K.

AU - Reiser, C. O.A.

AU - Berchtold, H.

AU - Storm, R.

AU - Hilgenfeld, R.

AU - Sprinzl, M.

PY - 1992/8/2

Y1 - 1992/8/2

N2 - Intact elongation factor Tu from Thermus thermophilus HB8 was crystallized in the presence of guanosine-5'-diphosphate (GDP), guanosine-5'-(β,γ-imido)triphosphate (GPPNP), guanosine-5'-(β,γ-methylene)triphosphate (GPPCP), p3-1-(2-nitro)phenylethylguanosine- 5'-triphosphate (caged GTP) and elongation factor Ts. An attempt to crystallize a ternary complex of EF-Tu·GPPNP with Phe-tRNAPhe was made. The crystals of EF-Tu obtained with GPPNP or GPPCP, respectively, were suitable for X-ray diffraction analysis at very high resolution.

AB - Intact elongation factor Tu from Thermus thermophilus HB8 was crystallized in the presence of guanosine-5'-diphosphate (GDP), guanosine-5'-(β,γ-imido)triphosphate (GPPNP), guanosine-5'-(β,γ-methylene)triphosphate (GPPCP), p3-1-(2-nitro)phenylethylguanosine- 5'-triphosphate (caged GTP) and elongation factor Ts. An attempt to crystallize a ternary complex of EF-Tu·GPPNP with Phe-tRNAPhe was made. The crystals of EF-Tu obtained with GPPNP or GPPCP, respectively, were suitable for X-ray diffraction analysis at very high resolution.

UR - https://www.scopus.com/pages/publications/0027109322

U2 - 10.1016/0022-0248(92)90270-S

DO - 10.1016/0022-0248(92)90270-S

M3 - Journal articles

AN - SCOPUS:0027109322

SN - 0022-0248

VL - 122

SP - 360

EP - 365

JO - Journal of Crystal Growth

JF - Journal of Crystal Growth

IS - 1-4

ER -

Crystals of intact elongation factor Tu from Thermus thermophilus diffracting to 1.45 Å resolution

Abstract

Funding

Research Areas and Centers

UN SDGs

Access to Document

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