Intact elongation factor Tu from Thermus thermophilus HB8 was crystallized in the presence of guanosine-5'-diphosphate (GDP), guanosine-5'-(β,γ-imido)triphosphate (GPPNP), guanosine-5'-(β,γ-methylene)triphosphate (GPPCP), p3-1-(2-nitro)phenylethylguanosine- 5'-triphosphate (caged GTP) and elongation factor Ts. An attempt to crystallize a ternary complex of EF-Tu·GPPNP with Phe-tRNAPhe was made. The crystals of EF-Tu obtained with GPPNP or GPPCP, respectively, were suitable for X-ray diffraction analysis at very high resolution.
Research Areas and Centers
- Academic Focus: Center for Infection and Inflammation Research (ZIEL)