TY - JOUR
T1 - Crystallization, structure solution and refinement of hen egg-white lysozyme at pH 8.0 in the presence of MPD
AU - Weiss, Manfred S.
AU - Palm, Gottfried J.
AU - Hilgenfeld, Rolf
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH using sodium chloride as the precipitant. However, the growth kinetics differ markedly between the two conditions. The major reason for this is a molecule of MPD that binds tightly in between two lysozyme molecules and favors the growth of the crystals along the crystallographic c direction over growth perpendicular to it.
AB - Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH using sodium chloride as the precipitant. However, the growth kinetics differ markedly between the two conditions. The major reason for this is a molecule of MPD that binds tightly in between two lysozyme molecules and favors the growth of the crystals along the crystallographic c direction over growth perpendicular to it.
UR - http://www.scopus.com/inward/record.url?scp=0033868957&partnerID=8YFLogxK
U2 - 10.1107/S0907444900006685
DO - 10.1107/S0907444900006685
M3 - Journal articles
C2 - 10944331
AN - SCOPUS:0033868957
SN - 0907-4449
VL - 56
SP - 952
EP - 958
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 8
ER -
