Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex

Guido Hansen; Timothy R. Hercus; Yibin Xu; Angel F. Lopez; Michael W. Parker; William J. McKinstry

doi:10.1107/S1744309108019404

Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex

Guido Hansen, Timothy R. Hercus, Yibin Xu, Angel F. Lopez, Michael W. Parker, William J. McKinstry

Institute of Biochemistry

5 Citations (Scopus)

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a haemopoietic growth factor that acts though a ternary receptor signalling complex containing specific α (GMRα) and common β (βc) receptor subunits. Human GM-CSF is encoded by the gene csf2, while the genes for GMRα and βc are csf2ra and csf2rb, respectively. Crystals of the ternary ectodomain complex comprising GM-CSF and the soluble extracellular regions of both the GMRα subunit and either βc or its glutamine-substitution mutant N346Q were obtained using the hanging-drop vapour-diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the βc subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P6 ₃22 and diffracted to 3.3 Å resolution.

Original language	English
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	64
Issue number	8
Pages (from-to)	711-714
Number of pages	4
ISSN	1744-3091
DOIs	https://doi.org/10.1107/S1744309108019404
Publication status	Published - 2008

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.1107/S1744309108019404

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abstract = "Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a haemopoietic growth factor that acts though a ternary receptor signalling complex containing specific α (GMRα) and common β (βc) receptor subunits. Human GM-CSF is encoded by the gene csf2, while the genes for GMRα and βc are csf2ra and csf2rb, respectively. Crystals of the ternary ectodomain complex comprising GM-CSF and the soluble extracellular regions of both the GMRα subunit and either βc or its glutamine-substitution mutant N346Q were obtained using the hanging-drop vapour-diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the βc subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P6 322 and diffracted to 3.3 {\AA} resolution.",

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AU - Hansen, Guido

AU - Hercus, Timothy R.

AU - Xu, Yibin

AU - Lopez, Angel F.

AU - Parker, Michael W.

AU - McKinstry, William J.

PY - 2008

Y1 - 2008

N2 - Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a haemopoietic growth factor that acts though a ternary receptor signalling complex containing specific α (GMRα) and common β (βc) receptor subunits. Human GM-CSF is encoded by the gene csf2, while the genes for GMRα and βc are csf2ra and csf2rb, respectively. Crystals of the ternary ectodomain complex comprising GM-CSF and the soluble extracellular regions of both the GMRα subunit and either βc or its glutamine-substitution mutant N346Q were obtained using the hanging-drop vapour-diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the βc subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P6 322 and diffracted to 3.3 Å resolution.

AB - Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a haemopoietic growth factor that acts though a ternary receptor signalling complex containing specific α (GMRα) and common β (βc) receptor subunits. Human GM-CSF is encoded by the gene csf2, while the genes for GMRα and βc are csf2ra and csf2rb, respectively. Crystals of the ternary ectodomain complex comprising GM-CSF and the soluble extracellular regions of both the GMRα subunit and either βc or its glutamine-substitution mutant N346Q were obtained using the hanging-drop vapour-diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the βc subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P6 322 and diffracted to 3.3 Å resolution.

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Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex

Abstract

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UN SDGs

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