TY - JOUR
T1 - Crystallization and preliminary crystallographic analysis of Rieske iron-sulfur protein II (soxF) from Sulfolobus acidocaldarius
AU - Bönisch, Heiko
AU - Schmidt, Christian L.
AU - Schäfer, Günter
AU - Ladenstein, Rudolf
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - An archaeal Rieske iron-sulfur protein has been crystallized for the first time. The genetically constructed soluble form of the soxF protein was expressed in E. coli. It contains a correctly inserted [2Fe-2S] cluster. The authentic soxF protein is part of a terminal oxidase complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius (DSM 639). The enzyme crystallizes in the space group P61 or P65, with unit-cell parameters a = b = 80.19, c = 75.69 Å. A complete data set has been collected to 1.64 Å resolution at 100 K.
AB - An archaeal Rieske iron-sulfur protein has been crystallized for the first time. The genetically constructed soluble form of the soxF protein was expressed in E. coli. It contains a correctly inserted [2Fe-2S] cluster. The authentic soxF protein is part of a terminal oxidase complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius (DSM 639). The enzyme crystallizes in the space group P61 or P65, with unit-cell parameters a = b = 80.19, c = 75.69 Å. A complete data set has been collected to 1.64 Å resolution at 100 K.
UR - http://www.scopus.com/inward/record.url?scp=12944277037&partnerID=8YFLogxK
U2 - 10.1107/S0907444900002456
DO - 10.1107/S0907444900002456
M3 - Journal articles
C2 - 10771436
AN - SCOPUS:12944277037
SN - 0907-4449
VL - 56
SP - 643
EP - 644
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 5
ER -
