TY - JOUR
T1 - Crystal structures of the X-domains of a Group-1 and a Group-3 coronavirus reveal that ADP-ribose-binding may not be a conserved property
AU - Piotrowski, Yvonne
AU - Hansen, Guido
AU - Boomaars-van Der Zanden, A. Linda
AU - Snijder, Eric J.
AU - Gorbalenya, Alexander E.
AU - Hilgenfeld, Rolf
PY - 2009/1/1
Y1 - 2009/1/1
N2 - The polyproteins of coronaviruses are cleaved by viral proteases into at least 15 nonstructural proteins (Nsps). Consisting of five domains, Nsp3 is the largest of these (180-210 kDa). Among these domains, the so-called X-domain is believed to act as ADP-ribose-1″-phosphate phosphatase or to bind poly(ADP-ribose). However, here we show that the X-domain of Infectious Bronchitis Virus (strain Beaudette), a Group-3 coronavirus, fails to bind ADP-ribose. This is explained on the basis of the crystal structure of the protein, determined at two different pH values. For comparison, we also describe the crystal structure of the homologous X-domain from Human Coronavirus 229E, a Group-1 coronavirus, which does bind ADP-ribose. Published by Wiley-Blackwell.
AB - The polyproteins of coronaviruses are cleaved by viral proteases into at least 15 nonstructural proteins (Nsps). Consisting of five domains, Nsp3 is the largest of these (180-210 kDa). Among these domains, the so-called X-domain is believed to act as ADP-ribose-1″-phosphate phosphatase or to bind poly(ADP-ribose). However, here we show that the X-domain of Infectious Bronchitis Virus (strain Beaudette), a Group-3 coronavirus, fails to bind ADP-ribose. This is explained on the basis of the crystal structure of the protein, determined at two different pH values. For comparison, we also describe the crystal structure of the homologous X-domain from Human Coronavirus 229E, a Group-1 coronavirus, which does bind ADP-ribose. Published by Wiley-Blackwell.
UR - http://www.scopus.com/inward/record.url?scp=58149466851&partnerID=8YFLogxK
U2 - 10.1002/pro.15
DO - 10.1002/pro.15
M3 - Journal articles
C2 - 19177346
AN - SCOPUS:58149466851
VL - 18
SP - 6
EP - 16
JO - Protein Science
JF - Protein Science
SN - 0961-8368
IS - 1
ER -