Crystal structure of the peroxo-diiron(III) intermediate of deoxyhypusine hydroxylase, an oxygenase involved in hypusination

Zhenggang Han, Naoki Sakai, Lars H. Böttger, Sebastián Klinke, Joachim Hauber, Alfred X. Trautwein, Rolf Hilgenfeld

Abstract

Deoxyhypusine hydroxylase (DOHH) is a non-heme diiron enzyme involved in the posttranslational modification of a critical lysine residue of eukaryotic translation initiation factor 5A (eIF-5A) to yield the unusual amino acid residue hypusine. This modification is essential for the role of eIF-5A in translation and in nuclear export of a group of specific mRNAs. The diiron center of human DOHH (hDOHH) forms a peroxo-diiron(III) intermediate (hDOHHperoxo) when its reduced form reacts with O2. hDOHHperoxo has a lifetime exceeding that of the peroxo intermediates of other diiron enzymes by several orders of magnitude. Here we report the 1.7-Å crystal structures of hDOHHperoxo and a complex with glycerol. The structure of hDOHHperoxo reveals the presence of a μ-1,2-peroxo-diiron(III) species at the active site. Augmented by UV/Vis and Mössbauer spectroscopic studies, the crystal structures offer explanations for the extreme longevity of hDOHHperoxo and illustrate how the enzyme specifically recognizes its only substrate, deoxyhypusine-eIF-5A.
Original languageEnglish
Title of host publicationStructure
Number of pages11
PublisherCell Press
Publication date05.05.2015
Pages882-892
ISBN (Print)1091-6490 (Electronic)\r0027-8424 (Linking)
DOIs
Publication statusPublished - 05.05.2015

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