Crystal structure of the middle domain of human poly(A)-binding protein-interacting protein 1

Jian Lei, Jeroen R. Mesters, Albrecht von Brunn, Rolf Hilgenfeld

Abstract

In eukaryotes, the poly(A)-binding protein (PABP) is one of the important factors for initiation of messenger RNA translation. PABP activity is regulated by the PABP-interacting proteins (Paips), which include Paip1, Paip2A, and Paip2B. Human Paip1 has three different isoforms. Here, we report the crystal structure of the middle domain of Paip1 isoform 2 (Paip1M) as determined by single-wavelength anomalous dispersion phasing. The structure reveals a crescent-shaped domain consisting of 10 α-helices and two antiparallel β-strands forming a β-hairpin. The 10 α-helices are arranged as five HEAT repeats which form a double layer of α helices with a convex and a concave surface. Despite low sequence identity, the overall fold of Paip1M is similar to the middle domain of human eIF4GII and yeast eIF4GI. Moreover, the amino-acid sequence motif and the local structure of eIF4G involved in binding of eIF4A, are conserved in Paip1. The structure reported here is the first of a member of the Paip family, thereby filling a gap in our understanding of initiation of eukaryotic mRNA translation in three dimensions. © 2011 Elsevier Inc.
Original languageEnglish
Title of host publicationBiochemical and Biophysical Research Communications
Number of pages6
Publication date20.05.2011
Pages680-685
ISBN (Print)1090-2104 (Electronic)\r0006-291X (Linking)
DOIs
Publication statusPublished - 20.05.2011

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  • HPLC/ESI Ion Trap Mass Spectrometer

    Hilgenfeld, R.

    01.05.0731.12.11

    Project: DFG ProjectsDFG Individual Projects

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