Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffinembedded bone tissue

Alireza Saraji, Anne Offermann, Janine Stegmann-Frehse, Katharina Hempel, Duan Kang, Rosemarie Krupar, Christian Watermann, Danny Jonigk, Mark Philipp Kuhnel, Jutta Kirfel, Sven Perner, Verena Sailer*

*Corresponding author for this work

Abstract

With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to the necessary decalcification process it was long thought that transcriptome analysis will not be feasible from decalcified formalin-fixed, paraffin-embedded (DFFPE) in a large manner. Here we demonstrate that mRNA extraction from DFFPE is feasible, quick, robust and reproducible and that decalcification does not hamper subsequent gene expression analysis. This might assist in implementing transcriptome analysis from DFFPE into every day practice.

Original languageEnglish
Article numbere0257416
JournalPLoS ONE
Volume16
Issue number9 September
DOIs
Publication statusPublished - 09.2021

Research Areas and Centers

  • Centers: University Cancer Center Schleswig-Holstein (UCCSH)

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