TY - JOUR
T1 - Coregistered Spectral Optical Coherence Tomography and Two-Photon Microscopy for Multimodal Near-Instantaneous Deep-Tissue Imaging
AU - Rakhymzhan, Asylkhan
AU - Reuter, Lucie
AU - Raspe, Raphael
AU - Bremer, Daniel
AU - Günther, Robert
AU - Leben, Ruth
AU - Heidelin, Judith
AU - Andresen, Volker
AU - Cheremukhin, Sergey
AU - Schulz-Hildebrandt, Hinnerk
AU - Bixel, Maria G.
AU - Adams, Ralf H.
AU - Radbruch, Helena
AU - Hüttmann, Gereon
AU - Hauser, Anja E.
AU - Niesner, Raluca A.
N1 - Funding Information:
We thank Peggy Mex for excellent technical support. Financial support from the German Ministry for Energy and Industry (ZIM project RETI-IM to R.A.N., V.A., and G.H.), and the German Research Council (DFG) under grant TRR130 (C01 to R.A.N. and A.E.H.; P17 to A.E.H. and H.R.), Ha5354/8-2 to A.E.H. and FOR2165/2 (Ni1167/4-2 to R.A.N. and Ha5354/6-2 to A.E.H.) is greatly acknowledged.
Publisher Copyright:
© 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Two-photon microscopy (2PM) has brought unique insight into the mechanisms underlying immune system dynamics and function since it enables monitoring of cellular motility and communication in complex systems within their genuine environment—the living organism. However, use of 2PM in clinical settings is limited. In contrast, optical coherence tomography (OCT), a noninvasive label-free diagnostic imaging method, which allows monitoring morphologic changes of large tissue regions in vivo, has found broad application in the clinic. Here we developed a combined multimodal technology to achieve near-instantaneous coregistered OCT, 2PM, and second harmonic generation (SHG) imaging over large volumes (up to 1,000 × 1,000 × 300 μm3) of tendons and other tissue compartments in mouse paws, as well as in mouse lymph nodes, spleens, and femurs. Using our multimodal imaging approach, we found differences in macrophage cell shape and motility behavior depending on whether they are located in tendons or in the surrounding tissue compartments of the mouse paw. The cellular shape of tissue-resident macrophages, indicative for their role in tissue, correlated with the supramolecular organization of collagen as revealed by SHG and OCT. Hence, the here-presented approach of coregistered OCT and 2PM has the potential to link specific cellular phenotypes and functions (as revealed by 2PM) to tissue morphology (as highlighted by OCT) and thus, to build a bridge between basic research knowledge and clinical observations.
AB - Two-photon microscopy (2PM) has brought unique insight into the mechanisms underlying immune system dynamics and function since it enables monitoring of cellular motility and communication in complex systems within their genuine environment—the living organism. However, use of 2PM in clinical settings is limited. In contrast, optical coherence tomography (OCT), a noninvasive label-free diagnostic imaging method, which allows monitoring morphologic changes of large tissue regions in vivo, has found broad application in the clinic. Here we developed a combined multimodal technology to achieve near-instantaneous coregistered OCT, 2PM, and second harmonic generation (SHG) imaging over large volumes (up to 1,000 × 1,000 × 300 μm3) of tendons and other tissue compartments in mouse paws, as well as in mouse lymph nodes, spleens, and femurs. Using our multimodal imaging approach, we found differences in macrophage cell shape and motility behavior depending on whether they are located in tendons or in the surrounding tissue compartments of the mouse paw. The cellular shape of tissue-resident macrophages, indicative for their role in tissue, correlated with the supramolecular organization of collagen as revealed by SHG and OCT. Hence, the here-presented approach of coregistered OCT and 2PM has the potential to link specific cellular phenotypes and functions (as revealed by 2PM) to tissue morphology (as highlighted by OCT) and thus, to build a bridge between basic research knowledge and clinical observations.
UR - http://www.scopus.com/inward/record.url?scp=85084379632&partnerID=8YFLogxK
U2 - 10.1002/cyto.a.24012
DO - 10.1002/cyto.a.24012
M3 - Journal articles
C2 - 32293804
AN - SCOPUS:85084379632
SN - 1552-4922
VL - 97
SP - 515
EP - 527
JO - Cytometry Part A
JF - Cytometry Part A
IS - 5
ER -