TY - JOUR
T1 - Conduits Mediate Transport of Low-Molecular-Weight Antigen to Lymph Node Follicles
AU - Roozendaal, Ramon
AU - Mempel, Thorsten R.
AU - Pitcher, Lisa A.
AU - Gonzalez, Santiago F.
AU - Verschoor, Admar
AU - Mebius, Reina E.
AU - von Andrian, Ulrich H.
AU - Carroll, Michael C.
N1 - Funding Information:
We wish to thank D. Vargas for exceptional animal care, R. Yeamans for technical assistance, and N. Barteneva at the IDI Flow and Imaging Cytometry Core facility and M. Ericsson at the Harvard Medical School EM Facility for technical assistance. R.R. is supported by a Marie Curie Fellowship (008873). M.C.C. is supported by a grant from the National Institutes of Health (AI 039246).
PY - 2009/2/20
Y1 - 2009/2/20
N2 - To track drainage of lymph-borne small and large antigens (Ags) into the peripheral lymph nodes and subsequent encounter by B cells and follicular dendritic cells, we used the approach of multiphoton intravital microscopy. We find a system of conduits that extend into the follicles and mediate delivery of small antigens to cognate B cells and follicular dendritic cells. The follicular conduits provide an efficient and rapid mechanism for delivery of small antigens and chemokines such as CXCL13 to B cells that directly contact the conduits. By contrast, large antigens were bound by subcapsular sinus macrophages and subsequently transferred to follicular B cells as previously reported. In summary, the findings identify a unique pathway for the channeling of small lymph-borne antigens and chemoattractants from the subcapsular sinus directly to the B cell follicles. This pathway could be used for enhancing delivery of vaccines or small molecules for improvement of humoral immunity.
AB - To track drainage of lymph-borne small and large antigens (Ags) into the peripheral lymph nodes and subsequent encounter by B cells and follicular dendritic cells, we used the approach of multiphoton intravital microscopy. We find a system of conduits that extend into the follicles and mediate delivery of small antigens to cognate B cells and follicular dendritic cells. The follicular conduits provide an efficient and rapid mechanism for delivery of small antigens and chemokines such as CXCL13 to B cells that directly contact the conduits. By contrast, large antigens were bound by subcapsular sinus macrophages and subsequently transferred to follicular B cells as previously reported. In summary, the findings identify a unique pathway for the channeling of small lymph-borne antigens and chemoattractants from the subcapsular sinus directly to the B cell follicles. This pathway could be used for enhancing delivery of vaccines or small molecules for improvement of humoral immunity.
UR - http://www.scopus.com/inward/record.url?scp=60149086845&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2008.12.014
DO - 10.1016/j.immuni.2008.12.014
M3 - Journal articles
C2 - 19185517
AN - SCOPUS:60149086845
SN - 1074-7613
VL - 30
SP - 264
EP - 276
JO - Immunity
JF - Immunity
IS - 2
ER -
