Abstract
Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.
| Original language | English |
|---|---|
| Journal | Nature Methods |
| Volume | 6 |
| Issue number | 8 |
| Pages (from-to) | 577-579 |
| Number of pages | 3 |
| ISSN | 1548-7091 |
| DOIs | |
| Publication status | Published - 07.07.2009 |
Funding
We thank T. Wandless (Stanford University) for providing the shield-1 ligand and plasmids encoding ddFKBP and S. Jonjic (University of Rijeka) for monoclonal antibody Croma 101. We thank P. Kay-Jackson for critical reading of the manuscript. This work was in part supported by a grant of the Deutsche Forschungsgemeinschaft (collaborative research grant 587, individual project A13).
Research Areas and Centers
- Research Area: Medical Genetics
