Conditional and reversible disruption of essential herpesvirus proteins

Mandy Glaß; Andreas Busche; Karen Wagner; Martin Messerle; Eva Maria Borst

doi:10.1038/nmeth.1346

Conditional and reversible disruption of essential herpesvirus proteins

Mandy Glaß, Andreas Busche, Karen Wagner, Martin Messerle^*, Eva Maria Borst

^*Corresponding author for this work

Institute of Human Genetics

Hannover Medical School

38 Citations (Scopus)

Abstract

Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

Original language	English
Journal	Nature Methods
Volume	6
Issue number	8
Pages (from-to)	577-579
Number of pages	3
ISSN	1548-7091
DOIs	https://doi.org/10.1038/nmeth.1346
Publication status	Published - 07.07.2009

Research Areas and Centers

Research Area: Medical Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/nmeth.1346

Cite this

@article{ffd7d33862994790b3242b01d02492ce,

title = "Conditional and reversible disruption of essential herpesvirus proteins",

abstract = "Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.",

author = "Mandy Gla{\ss} and Andreas Busche and Karen Wagner and Martin Messerle and Borst, {Eva Maria}",

year = "2009",

month = jul,

day = "7",

doi = "10.1038/nmeth.1346",

language = "English",

volume = "6",

pages = "577--579",

journal = "Nature Methods",

issn = "1548-7091",

number = "8",

}

TY - JOUR

T1 - Conditional and reversible disruption of essential herpesvirus proteins

AU - Glaß, Mandy

AU - Busche, Andreas

AU - Wagner, Karen

AU - Messerle, Martin

AU - Borst, Eva Maria

PY - 2009/7/7

Y1 - 2009/7/7

N2 - Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

AB - Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

UR - http://www.scopus.com/inward/record.url?scp=68349116137&partnerID=8YFLogxK

U2 - 10.1038/nmeth.1346

DO - 10.1038/nmeth.1346

M3 - Journal articles

C2 - 19578384

AN - SCOPUS:68349116137

SN - 1548-7091

VL - 6

SP - 577

EP - 579

JO - Nature Methods

JF - Nature Methods

IS - 8

ER -

Conditional and reversible disruption of essential herpesvirus proteins

Abstract

Research Areas and Centers

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this