Comprehensive methylation analysis in typical and atypical PWS and AS patients with normal biparental chromosomes 15

Maren Runte, Claudia Färber, Christina Lich, Michael Zeschnigk, Tina Buchholz, Arabella Smith, Lionel Van Maldergem, Joachim Bürger, Françoise Muscatelli, Gabriele Gillessen-Kaesbach, Bernhard Horsthemke*, Karin Buiting

*Corresponding author for this work
12 Citations (Scopus)


Imprinting defects in 15q11-q13 are a rare but significant cause of Prader-Willi syndrome (PWS) and Angelman syndrome (AS). Patients with an imprinting defect have apparently normal chromosomes 15 of biparental origin, but are recognised by uniparental DNA methylation at D15S63 (PW71) or SNURF-SNRPN exon 1. We have investigated the methylation status of five additional loci in 12 such patients with or without a deletion in the imprinting centre. In each patient, the imprinting defect affected all loci tested. During routine diagnostic testing we identified four patients who had a normal methylation pattern at SNURF-SNRPN exon 1, but an abnormal pattern at D15S63. In two of these patients, who were suspected of having PWS, this change was restricted to D15S63. In two patients suspected of having AS, several but not all loci were affected. Using a newly developed methylation-specific PCR test for D15S63 we found that these methylation changes are rare in patients suspected of having AS. Although we can not prove that the methylation changes in the four patients are causally related to their disease, our findings demonstrate that spatially restricted changes in methylation can occur. In some cases, these changes may reflect incomplete imprint spreading.

Original languageEnglish
JournalEuropean Journal of Human Genetics
Issue number7
Pages (from-to)519-526
Number of pages8
Publication statusPublished - 2001

Research Areas and Centers

  • Research Area: Medical Genetics


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