Projects per year
Abstract
Human blood group A and B glycosyltransferases (GTA, GTB) are highly homologous glycosyltransferases. A number of high-resolution crystal structures is available showing that these enzymes convert from an open conformation into a catalytically active closed conformation upon substrate binding. However, the mechanism of glycosyltransfer is still under debate, and the precise nature as well as the time scales of conformational transitions are unknown. NMR offers a variety of experiments to shine more light on these unresolved questions. Therefore, in a first step we have assigned all methyl resonance signals in MILVA labeled samples of GTA and GTB, still a challenging task for 70 kDa homodimeric proteins. Assignments were obtained from methyl–methyl NOESY experiments, and from measurements of lanthanide-induced pseudocontact shifts (PCS) using high resolution crystal structures as templates. PCSs and chemical shift perturbations, induced by substrate analogue binding, suggest that the fully closed state is not adopted in the presence of lanthanide ions.
Original language | English |
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Journal | Journal of Biomolecular NMR |
Volume | 70 |
Issue number | 4 |
Pages (from-to) | 245-259 |
Number of pages | 15 |
ISSN | 0925-2738 |
DOIs | |
Publication status | Published - 01.04.2018 |
Research Areas and Centers
- Academic Focus: Center for Infection and Inflammation Research (ZIEL)
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Dive into the research topics of 'Complete assignment of Ala, Ile, Leu, Met and Val methyl groups of human blood group A and B glycosyltransferases using lanthanide-induced pseudocontact shifts and methyl–methyl NOESY'. Together they form a unique fingerprint.Projects
- 1 Finished
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Protein dynamics and substrate recognition of human blood group glycosyltransferases
Peters, T. (Principal Investigator (PI))
01.01.14 → 01.01.18
Project: DFG Projects › DFG Individual Projects