Combined treatment of breast cancer cell lines with vitamin D and COX-2 inhibitors

Marc Thill; Kathrin Reichert; Alena Woeste; Stephan Polack; Dorothea Fischer; Friederike Hoellen; Achim Rody; Michael Friedrich; Frank Köster

Combined treatment of breast cancer cell lines with vitamin D and COX-2 inhibitors

Marc Thill, Kathrin Reichert, Alena Woeste, Stephan Polack, Dorothea Fischer, Friederike Hoellen, Achim Rody, Michael Friedrich, Frank Köster^*

^*Corresponding author for this work

Clinic of Gynecology and Obstetrics

13 Citations (Scopus)

Abstract

Background: Vitamin D is known for its anticancerogenous potential. Prostaglandin E₂ (PGE₂) is a proliferation and inflammation activating agent. The production of PGE₂ is dependent on the activity of cyclooxygenase-2 (COX-2). A link between vitamin D and PGE₂ metabolism was recently shown. Materials and Methods: In MDA-MB-231 and MCF-7 breast cancer cell lines we investigated the influence of calcitriol and the COX-2 inhibitor celecoxib regarding cell growth via MTT test, as well as on the protein and mRNA expression of COX-2 using western blot and qRT-PCR. Results: The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX-2 inhibitor celecoxib and even stronger by their combination. Moreover, calcitriol inhibited the COX-2 protein expression in MDA-MB-231, as well as the COX-2 mRNA expression in both cell lines. Conclusion: The combination of calcitriol and celecoxib demonstrated a cooperative growth-inhibiting effect in breast cancer cell lines.

Original language	English
Journal	Anticancer Research
Volume	35
Issue number	2
Pages (from-to)	1189-1195
Number of pages	7
ISSN	0250-7005
Publication status	Published - 01.01.2015

Research Areas and Centers

Research Area: Luebeck Integrated Oncology Network (LION)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Combined treatment of breast cancer cell lines with vitamin D and COX-2 inhibitors",

abstract = "Background: Vitamin D is known for its anticancerogenous potential. Prostaglandin E2 (PGE2) is a proliferation and inflammation activating agent. The production of PGE2 is dependent on the activity of cyclooxygenase-2 (COX-2). A link between vitamin D and PGE2 metabolism was recently shown. Materials and Methods: In MDA-MB-231 and MCF-7 breast cancer cell lines we investigated the influence of calcitriol and the COX-2 inhibitor celecoxib regarding cell growth via MTT test, as well as on the protein and mRNA expression of COX-2 using western blot and qRT-PCR. Results: The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX-2 inhibitor celecoxib and even stronger by their combination. Moreover, calcitriol inhibited the COX-2 protein expression in MDA-MB-231, as well as the COX-2 mRNA expression in both cell lines. Conclusion: The combination of calcitriol and celecoxib demonstrated a cooperative growth-inhibiting effect in breast cancer cell lines.",

author = "Marc Thill and Kathrin Reichert and Alena Woeste and Stephan Polack and Dorothea Fischer and Friederike Hoellen and Achim Rody and Michael Friedrich and Frank K{\"o}ster",

year = "2015",

month = jan,

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language = "English",

volume = "35",

pages = "1189--1195",

journal = "Anticancer Research",

issn = "0250-7005",

number = "2",

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TY - JOUR

T1 - Combined treatment of breast cancer cell lines with vitamin D and COX-2 inhibitors

AU - Thill, Marc

AU - Reichert, Kathrin

AU - Woeste, Alena

AU - Polack, Stephan

AU - Fischer, Dorothea

AU - Hoellen, Friederike

AU - Rody, Achim

AU - Friedrich, Michael

AU - Köster, Frank

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Background: Vitamin D is known for its anticancerogenous potential. Prostaglandin E2 (PGE2) is a proliferation and inflammation activating agent. The production of PGE2 is dependent on the activity of cyclooxygenase-2 (COX-2). A link between vitamin D and PGE2 metabolism was recently shown. Materials and Methods: In MDA-MB-231 and MCF-7 breast cancer cell lines we investigated the influence of calcitriol and the COX-2 inhibitor celecoxib regarding cell growth via MTT test, as well as on the protein and mRNA expression of COX-2 using western blot and qRT-PCR. Results: The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX-2 inhibitor celecoxib and even stronger by their combination. Moreover, calcitriol inhibited the COX-2 protein expression in MDA-MB-231, as well as the COX-2 mRNA expression in both cell lines. Conclusion: The combination of calcitriol and celecoxib demonstrated a cooperative growth-inhibiting effect in breast cancer cell lines.

AB - Background: Vitamin D is known for its anticancerogenous potential. Prostaglandin E2 (PGE2) is a proliferation and inflammation activating agent. The production of PGE2 is dependent on the activity of cyclooxygenase-2 (COX-2). A link between vitamin D and PGE2 metabolism was recently shown. Materials and Methods: In MDA-MB-231 and MCF-7 breast cancer cell lines we investigated the influence of calcitriol and the COX-2 inhibitor celecoxib regarding cell growth via MTT test, as well as on the protein and mRNA expression of COX-2 using western blot and qRT-PCR. Results: The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX-2 inhibitor celecoxib and even stronger by their combination. Moreover, calcitriol inhibited the COX-2 protein expression in MDA-MB-231, as well as the COX-2 mRNA expression in both cell lines. Conclusion: The combination of calcitriol and celecoxib demonstrated a cooperative growth-inhibiting effect in breast cancer cell lines.

UR - http://www.scopus.com/inward/record.url?scp=84922880604&partnerID=8YFLogxK

M3 - Journal articles

C2 - 25667510

AN - SCOPUS:84922880604

SN - 0250-7005

VL - 35

SP - 1189

EP - 1195

JO - Anticancer Research

JF - Anticancer Research

IS - 2

ER -

Combined treatment of breast cancer cell lines with vitamin D and COX-2 inhibitors

Abstract

Research Areas and Centers

UN SDGs

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