Combined treatment of breast cancer cell lines with vitamin D and COX-2 inhibitors

Marc Thill, Kathrin Reichert, Alena Woeste, Stephan Polack, Dorothea Fischer, Friederike Hoellen, Achim Rody, Michael Friedrich, Frank Köster*

*Corresponding author for this work
13 Citations (Scopus)


Background: Vitamin D is known for its anticancerogenous potential. Prostaglandin E2 (PGE2) is a proliferation and inflammation activating agent. The production of PGE2 is dependent on the activity of cyclooxygenase-2 (COX-2). A link between vitamin D and PGE2 metabolism was recently shown. Materials and Methods: In MDA-MB-231 and MCF-7 breast cancer cell lines we investigated the influence of calcitriol and the COX-2 inhibitor celecoxib regarding cell growth via MTT test, as well as on the protein and mRNA expression of COX-2 using western blot and qRT-PCR. Results: The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX-2 inhibitor celecoxib and even stronger by their combination. Moreover, calcitriol inhibited the COX-2 protein expression in MDA-MB-231, as well as the COX-2 mRNA expression in both cell lines. Conclusion: The combination of calcitriol and celecoxib demonstrated a cooperative growth-inhibiting effect in breast cancer cell lines.

Original languageEnglish
JournalAnticancer Research
Issue number2
Pages (from-to)1189-1195
Number of pages7
Publication statusPublished - 01.01.2015

Research Areas and Centers

  • Research Area: Luebeck Integrated Oncology Network (LION)


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