TY - JOUR
T1 - Collagen analysis of the ascending aortic dilatation associated with bicuspid aortic valve disease compared with tricuspid aortic valve
AU - Navarrete Santos, Alexander
AU - Yan, Junfeng
AU - Lochmann, Peter
AU - Pfeil, Heike
AU - Petersen, Michael
AU - Simm, Andreas
AU - Treede, Hendrik
AU - Sievers, Hans H.
AU - Mohamed, Salah A.
N1 - Publisher Copyright:
© 2016 Informa UK Limited, trading as Taylor & Francis Group.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2016/10/19
Y1 - 2016/10/19
N2 - Dilatation of the ascending aorta is a common occurrence in patients with bicuspid aortic valve (BAV). The aim of the current study was to characterize collagen content in advanced glycation end products (AGEs) of dilated aortic tissue from two distinct areas, concave and convex aortic sites in patients with BAV and TAV. Collagen contents extracted from 100 mg tissue was isolated by enzymatic digestion using pepsin and the nondigested material was further digested using cyanogen bromide, insoluble collagen fraction (ICF) was extracted by hydrochloric acid hydrolysis. BAV tissue showed diminished fluorescence of the pepsin extracted fraction (PEF) compared with TAV tissue (12.4 ± 1.0% vs 32.9 ± 7.6%, p = 0.05). Patients with BAV had PEF of collagens significantly diminished in the dilated ascending aorta, especially in its convex portion, in course of aging and increment of dilated diameters. It is suggestible that BAV patients present more highly AGE-modified collagens in their ascending aorta.
AB - Dilatation of the ascending aorta is a common occurrence in patients with bicuspid aortic valve (BAV). The aim of the current study was to characterize collagen content in advanced glycation end products (AGEs) of dilated aortic tissue from two distinct areas, concave and convex aortic sites in patients with BAV and TAV. Collagen contents extracted from 100 mg tissue was isolated by enzymatic digestion using pepsin and the nondigested material was further digested using cyanogen bromide, insoluble collagen fraction (ICF) was extracted by hydrochloric acid hydrolysis. BAV tissue showed diminished fluorescence of the pepsin extracted fraction (PEF) compared with TAV tissue (12.4 ± 1.0% vs 32.9 ± 7.6%, p = 0.05). Patients with BAV had PEF of collagens significantly diminished in the dilated ascending aorta, especially in its convex portion, in course of aging and increment of dilated diameters. It is suggestible that BAV patients present more highly AGE-modified collagens in their ascending aorta.
UR - http://www.scopus.com/inward/record.url?scp=84990879584&partnerID=8YFLogxK
U2 - 10.1080/13813455.2016.1211710
DO - 10.1080/13813455.2016.1211710
M3 - Journal articles
C2 - 27595307
AN - SCOPUS:84990879584
SN - 1381-3455
VL - 122
SP - 289
EP - 294
JO - Archives of Physiology and Biochemistry
JF - Archives of Physiology and Biochemistry
IS - 5
ER -