TY - JOUR
T1 - CMP-N-acetylneuraminic acid hydroxylase: The first cytosolic Rieske iron-sulphur protein to be described in Eukarya
AU - Schlenzka, Wiebke
AU - Shaw, Lee
AU - Kelm, Soerge
AU - Schmidt, Christian L.
AU - Bill, Eckhard
AU - Trautwein, Alfred X.
AU - Lottspeich, Friedrich
AU - Schauer, Roland
PY - 1996/5/6
Y1 - 1996/5/6
N2 - Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-N-acetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known to be characteristic for a Rieske iron-sulphur centre (2Fe-2S), the g-values being 1.78, 1.91 and 2.01, respectively. An analysis of the primary structure of the hydroxylase led to the identification of an amino acid sequence, known to be characteristic for Rieske proteins. Furthermore, possible binding sites for cytochrome b5, the substrate CMPNeu5Ac and a mononuclear iron centre were also identified.
AB - Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-N-acetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known to be characteristic for a Rieske iron-sulphur centre (2Fe-2S), the g-values being 1.78, 1.91 and 2.01, respectively. An analysis of the primary structure of the hydroxylase led to the identification of an amino acid sequence, known to be characteristic for Rieske proteins. Furthermore, possible binding sites for cytochrome b5, the substrate CMPNeu5Ac and a mononuclear iron centre were also identified.
UR - http://www.scopus.com/inward/record.url?scp=0029941677&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(96)00384-5
DO - 10.1016/0014-5793(96)00384-5
M3 - Journal articles
C2 - 8647250
AN - SCOPUS:0029941677
SN - 0014-5793
VL - 385
SP - 197
EP - 200
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -
