Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia

H. von Goessel; U. Jacobs; S. Semper; M. Krumbholz; T. Langer; T. Keller; A. Schrauder; V. H.J. van der Velden; J. J.M. van Dongen; J. Harbott; E. R. Panzer-Grümayer; M. Schrappe; W. Rascher; M. Metzler

doi:10.1016/j.leukres.2008.11.001

Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia

H. von Goessel, U. Jacobs, S. Semper, M. Krumbholz, T. Langer, T. Keller, A. Schrauder, V. H.J. van der Velden, J. J.M. van Dongen, J. Harbott, E. R. Panzer-Grümayer, M. Schrappe, W. Rascher, M. Metzler^*

^*Corresponding author for this work

Clinic of Pediatric and Adolescent Medicine

20 Citations (Scopus)

Abstract

Fusion between ETV6 and RUNX1 defines the largest genetic subgroup in childhood ALL. The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease. Sequencing of DNA breakpoints has been difficult due to the extended size of the respective breakpoint cluster regions. We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use. Resulting fusion sites and breakpoints derived from previous studies were subject to cluster analysis to identify potential sequence motifs involved in translocation initiation.

Original language	English
Journal	Leukemia Research
Volume	33
Issue number	8
Pages (from-to)	1082-1088
Number of pages	7
ISSN	0145-2126
DOIs	https://doi.org/10.1016/j.leukres.2008.11.001
Publication status	Published - 08.2009

Funding

This study was supported by a Wilhelm-Sander Foundation grant to MM and TL. HvG was supported by the ELAN program of the University of Erlangen. ERP-G was received grants by the ÖNB Jubilaeumsfond, FWF and GENAU-CHILD Projekt.

Research Areas and Centers

Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.leukres.2008.11.001

Cite this

von Goessel, H., Jacobs, U., Semper, S., Krumbholz, M., Langer, T., Keller, T., Schrauder, A., van der Velden, V. H. J., van Dongen, J. J. M., Harbott, J., Panzer-Grümayer, E. R., Schrappe, M., Rascher, W., & Metzler, M. (2009). Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia. Leukemia Research, 33(8), 1082-1088. https://doi.org/10.1016/j.leukres.2008.11.001

@article{dc7a83b8eaf4485ca766a6ee2d3301fb,

title = "Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia",

abstract = "Fusion between ETV6 and RUNX1 defines the largest genetic subgroup in childhood ALL. The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease. Sequencing of DNA breakpoints has been difficult due to the extended size of the respective breakpoint cluster regions. We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use. Resulting fusion sites and breakpoints derived from previous studies were subject to cluster analysis to identify potential sequence motifs involved in translocation initiation.",

author = "\{von Goessel\}, H. and U. Jacobs and S. Semper and M. Krumbholz and T. Langer and T. Keller and A. Schrauder and \{van der Velden\}, \{V. H.J.\} and \{van Dongen\}, \{J. J.M.\} and J. Harbott and Panzer-Gr{\"u}mayer, \{E. R.\} and M. Schrappe and W. Rascher and M. Metzler",

note = "Funding Information: This study was supported by a Wilhelm-Sander Foundation grant to MM and TL. HvG was supported by the ELAN program of the University of Erlangen. ERP-G was received grants by the {\"O}NB Jubilaeumsfond, FWF and GENAU-CHILD Projekt. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2009",

month = aug,

doi = "10.1016/j.leukres.2008.11.001",

language = "English",

volume = "33",

pages = "1082--1088",

journal = "Leukemia Research",

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publisher = "Elsevier Ltd",

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von Goessel, H, Jacobs, U, Semper, S, Krumbholz, M, Langer, T, Keller, T, Schrauder, A, van der Velden, VHJ, van Dongen, JJM, Harbott, J, Panzer-Grümayer, ER, Schrappe, M, Rascher, W & Metzler, M 2009, 'Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia', Leukemia Research, vol. 33, no. 8, pp. 1082-1088. https://doi.org/10.1016/j.leukres.2008.11.001

TY - JOUR

T1 - Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia

AU - von Goessel, H.

AU - Jacobs, U.

AU - Semper, S.

AU - Krumbholz, M.

AU - Langer, T.

AU - Keller, T.

AU - Schrauder, A.

AU - van der Velden, V. H.J.

AU - van Dongen, J. J.M.

AU - Harbott, J.

AU - Panzer-Grümayer, E. R.

AU - Schrappe, M.

AU - Rascher, W.

AU - Metzler, M.

N1 - Funding Information: This study was supported by a Wilhelm-Sander Foundation grant to MM and TL. HvG was supported by the ELAN program of the University of Erlangen. ERP-G was received grants by the ÖNB Jubilaeumsfond, FWF and GENAU-CHILD Projekt. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2009/8

Y1 - 2009/8

N2 - Fusion between ETV6 and RUNX1 defines the largest genetic subgroup in childhood ALL. The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease. Sequencing of DNA breakpoints has been difficult due to the extended size of the respective breakpoint cluster regions. We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use. Resulting fusion sites and breakpoints derived from previous studies were subject to cluster analysis to identify potential sequence motifs involved in translocation initiation.

AB - Fusion between ETV6 and RUNX1 defines the largest genetic subgroup in childhood ALL. The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease. Sequencing of DNA breakpoints has been difficult due to the extended size of the respective breakpoint cluster regions. We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use. Resulting fusion sites and breakpoints derived from previous studies were subject to cluster analysis to identify potential sequence motifs involved in translocation initiation.

UR - https://www.scopus.com/pages/publications/67349202133

U2 - 10.1016/j.leukres.2008.11.001

DO - 10.1016/j.leukres.2008.11.001

M3 - Journal articles

C2 - 19081626

AN - SCOPUS:67349202133

SN - 0145-2126

VL - 33

SP - 1082

EP - 1088

JO - Leukemia Research

JF - Leukemia Research

IS - 8

ER -

Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia

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Research Areas and Centers

UN SDGs

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