Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Jeanette Erdmann; Sabine Hassfeld; Heike Kallisch; Eckart Fleck; Vera Regitz-Zagrosek

doi:10.1006/bbrc.1998.8311

Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Jeanette Erdmann^*, Sabine Hassfeld, Heike Kallisch, Eckart Fleck, Vera Regitz-Zagrosek

^*Corresponding author for this work

Institute of Cardiogenetics

Charite - Universitatsmedizin Berlin

10 Citations (Scopus)

Abstract

To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	244
Issue number	2
Pages (from-to)	494-497
Number of pages	4
ISSN	0006-291X
DOIs	https://doi.org/10.1006/bbrc.1998.8311
Publication status	Published - 17.03.1998

Funding

This study was supported in part by a grant of the Verbund Klin-ische Pharmakologie Berlin/Brandenburg.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/bbrc.1998.8311

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title = "Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene",

abstract = "To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.",

author = "Jeanette Erdmann and Sabine Hassfeld and Heike Kallisch and Eckart Fleck and Vera Regitz-Zagrosek",

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T1 - Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

AU - Erdmann, Jeanette

AU - Hassfeld, Sabine

AU - Kallisch, Heike

AU - Fleck, Eckart

AU - Regitz-Zagrosek, Vera

PY - 1998/3/17

Y1 - 1998/3/17

N2 - To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.

AB - To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.

UR - https://www.scopus.com/pages/publications/0032539769

U2 - 10.1006/bbrc.1998.8311

DO - 10.1006/bbrc.1998.8311

M3 - Journal articles

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EP - 497

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Abstract

Funding

UN SDGs

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