Chromosomal aneuploidy affects the global proteome equilibrium of colorectal cancer cells

Timo Gemoll*, Jens K. Habermann, Susanne Becker, Silke Szymczak, Madhvi B. Upender, Hans Peter Bruch, Ulf Hellman, Thomas Ried, Gert Auer, Hans Jörnvall, Uwe J. Roblick

*Corresponding author for this work
9 Citations (Scopus)


Background: Chromosomal aneuploidy has been identified as a prognostic factor in the majority of sporadic carcinomas. However, it is not known how chromosomal aneuploidy affects chromosome-specific protein expression in particular, and the cellular proteome equilibrium in general. Objective: The aim was to detect chromosomal aneuploidy-associated expression changes in cell clones carrying trisomies found in colorectal cancer. methods: We used microcell-mediated chromosomal transfer to generate three artificial trisomic cell clones of the karyotypically stable, diploid, yet mismatch-deficient, colorectal cancer cell line DLD1 - each of them harboring one extra copy of either chromosome 3, 7 or 13. Protein expression differences were assessed by two-dimensional gel electrophoresis and mass spectrometry, compared to whole-genome gene expression data, and evaluated by PANTHER classification system and Ingenuity Pathway Analysis (IPA). RESULTS: In total, 79 differentially expressed proteins were identified between the trisomic clones and the parental cell line. Up-regulation of PCNA and HMGB1 as well as down-regulation of IDH3A and PSMB3 were revealed as trisomy-associated alterations involved in regulating genome stability. CONCLUSIONS: These results show that trisomies affect the expression of genes and proteins that are not necessarily located on the trisomic chromosome, but reflect a pathway-related alteration of the cellular equilibrium.

Original languageEnglish
JournalAnalytical Cellular Pathology
Issue number5-6
Pages (from-to)149-161
Number of pages13
Publication statusPublished - 01.01.2013


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