TY - JOUR
T1 - Chlorin-Based Photoactivable Galectin-3-Inhibitor Nanoliposome for Enhanced Photodynamic Therapy and NK Cell-Related Immunity in Melanoma
AU - Wang, Sijia
AU - Liu, Huifang
AU - Xin, Jing
AU - Rahmanzadeh, Ramtin
AU - Wang, Jing
AU - Yao, Cuiping
AU - Zhang, Zhenxi
N1 - Funding Information:
The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. This work was supported by the National Natural Science Foundation of China under grant nos. 61705177, 61727823, 61875159, and 61775178, and by the China Postdoctoral Science Foundation under grant nos. 2018T111036 and 2017M613107. The authors declare no competing financial interest.
Publisher Copyright:
Copyright © 2019 American Chemical Society.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/11/13
Y1 - 2019/11/13
N2 - Photodynamic therapy (PDT) is an encouraging alternative therapy for melanoma treatment and Ce6-mediated PDT has shown some exciting results in clinical trials. However, PDT in melanoma treatment is still hampered by some melanoma's protective mechanisms like antiapoptosis mechanisms and treatment escape pathways. Combined therapy and enhancing immune stimulation were proposed as effective strategies to overcome this resistance. In this paper, a Chlorin-based photoactivable Galectin-3-inhibitor nanoliposome (PGIL) was designed for enhanced Melanoma PDT and immune activation of Natural Killer (NK) cells. PGIL were synthesized by encapsulating the photosensitizer chlorin e6 and low molecular citrus pectin in the nanoliposome to realize NIR-triggered PDT and low molecular citrus pectin (LCP) release into the cytoplasm. The intracellular release of LCP inhibits the activity of galectin-3, which increases the apoptosis, inhibits the invade ability, and enhances the recognition ability of Natural Killer (NK) cells to tumor cells in melanoma cells after PDT. These effects of PGIL were tested in cells and nude mice, and the mechanisms during the in vivo treatment were preliminarily studied. The results showed that PGIL can be an effective prodrug for melanoma therapy.
AB - Photodynamic therapy (PDT) is an encouraging alternative therapy for melanoma treatment and Ce6-mediated PDT has shown some exciting results in clinical trials. However, PDT in melanoma treatment is still hampered by some melanoma's protective mechanisms like antiapoptosis mechanisms and treatment escape pathways. Combined therapy and enhancing immune stimulation were proposed as effective strategies to overcome this resistance. In this paper, a Chlorin-based photoactivable Galectin-3-inhibitor nanoliposome (PGIL) was designed for enhanced Melanoma PDT and immune activation of Natural Killer (NK) cells. PGIL were synthesized by encapsulating the photosensitizer chlorin e6 and low molecular citrus pectin in the nanoliposome to realize NIR-triggered PDT and low molecular citrus pectin (LCP) release into the cytoplasm. The intracellular release of LCP inhibits the activity of galectin-3, which increases the apoptosis, inhibits the invade ability, and enhances the recognition ability of Natural Killer (NK) cells to tumor cells in melanoma cells after PDT. These effects of PGIL were tested in cells and nude mice, and the mechanisms during the in vivo treatment were preliminarily studied. The results showed that PGIL can be an effective prodrug for melanoma therapy.
UR - http://www.scopus.com/inward/record.url?scp=85074910288&partnerID=8YFLogxK
U2 - 10.1021/acsami.9b09560
DO - 10.1021/acsami.9b09560
M3 - Journal articles
C2 - 31617343
AN - SCOPUS:85074910288
SN - 1944-8244
VL - 11
SP - 41829
EP - 41841
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 45
ER -
