TY - JOUR
T1 - Chimeric hepatitis A virus particles presenting a foreign epitope (HIV gp41) at their surface
AU - Beneduce, Francesca
AU - Kusov, Yuri
AU - Klinger, Matthias
AU - Gauss-Müller, Verena
AU - Morace, Graziella
N1 - Funding Information:
The authors thank Professor H. Katinger for kindly providing the human monoclonal antibody CHO2F5. This work was supported by grants from the ‘Viral Hepatitis Project’ and ‘Viral Vaccines Project’ of the Istituto Superiore di Sanità and by the Deutsche Forschungsgemeinschaft (Transfer project 24).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002
Y1 - 2002
N2 - Hepatitis A virus (HAV) protein 2A has been demonstrated to be involved in virus morphogenesis and suggested to be located on the surface of the particle. To determine whether this protein can function as a target structure to harbor and expose foreign epitopes on HAV particles, a full-length HAV cDNA, containing a seven amino acid stretch of human immunodeficiency virus type 1 (HIV-1) envelope protein gp41, was constructed. Following vaccinia virus MVA-T7-mediated expression of the cDNA in COS7 and Huh-T7 cells, chimeric HAV particles, exposing the foreign epitope gp41 on their surface, were produced. These particles were found to be empty capsids (70S), as judged by immunospecific enzyme linked immunosorbent assay (ELISA) on sucrose gradient fractions and immunoelectron microscopy. The immunological detection of VP1-2A harboring the gp41 epitope of HIV suggests that the 2A domain of HAV is suitable to present foreign antigenic epitopes.
AB - Hepatitis A virus (HAV) protein 2A has been demonstrated to be involved in virus morphogenesis and suggested to be located on the surface of the particle. To determine whether this protein can function as a target structure to harbor and expose foreign epitopes on HAV particles, a full-length HAV cDNA, containing a seven amino acid stretch of human immunodeficiency virus type 1 (HIV-1) envelope protein gp41, was constructed. Following vaccinia virus MVA-T7-mediated expression of the cDNA in COS7 and Huh-T7 cells, chimeric HAV particles, exposing the foreign epitope gp41 on their surface, were produced. These particles were found to be empty capsids (70S), as judged by immunospecific enzyme linked immunosorbent assay (ELISA) on sucrose gradient fractions and immunoelectron microscopy. The immunological detection of VP1-2A harboring the gp41 epitope of HIV suggests that the 2A domain of HAV is suitable to present foreign antigenic epitopes.
UR - http://www.scopus.com/inward/record.url?scp=0036294324&partnerID=8YFLogxK
U2 - 10.1016/S0166-3542(02)00073-6
DO - 10.1016/S0166-3542(02)00073-6
M3 - Journal articles
C2 - 12103436
AN - SCOPUS:0036294324
SN - 0166-3542
VL - 55
SP - 369
EP - 377
JO - Antiviral Research
JF - Antiviral Research
IS - 2
ER -
