TY - JOUR
T1 - Characterization of WNT7A expression in human endometrium and endometriotic lesions
AU - Gaetje, Regine
AU - Holtrich, Uwe
AU - Karn, Thomas
AU - Cikrit, Eva
AU - Engels, Knut
AU - Rody, Achim
AU - Kaufmann, Manfred
N1 - Funding Information:
Supported by grants from Deutsche Krebshilfe, Bonn Margarete Bonifer-Stiftung, Bad Soden, BANSS-Stiftung, Biedenkopf, and Dr. Robert Pfleger-Stiftung, Bamberg.
PY - 2007/12
Y1 - 2007/12
N2 - Objective: To characterize the expression of WNT7A in human eutopic and ectopic endometrium. Design: Experimental study using real-time polymerase chain reaction, laser microdissection, in situ hybridization, and immunofluorescence. Setting: University-based laboratory. Patient(s): Patients with and without endometriosis undergoing surgery for benign indications. Intervention(s): None. Main Outcome Measure(s): Relative expression values compared with housekeeping genes using real-time polymerase chain reaction. Detection of positive cells by immunofluorescence and in situ hybridization. Result(s): In endometriosis, statistically significant higher WNT7A mRNA expression was observed compared with eutopic endometrium. Expression of WNT7A was found in the luminal and glandular epithelial cells as well as stroma cells in endometrium and endometriosis by immunofluorescence, in situ hybridization, and polymerase chain reaction of laser microdissected tissue. Conclusion(s): The results of the present study suggest that WNT7A plays a role in the pathophysiology of endometriosis.
AB - Objective: To characterize the expression of WNT7A in human eutopic and ectopic endometrium. Design: Experimental study using real-time polymerase chain reaction, laser microdissection, in situ hybridization, and immunofluorescence. Setting: University-based laboratory. Patient(s): Patients with and without endometriosis undergoing surgery for benign indications. Intervention(s): None. Main Outcome Measure(s): Relative expression values compared with housekeeping genes using real-time polymerase chain reaction. Detection of positive cells by immunofluorescence and in situ hybridization. Result(s): In endometriosis, statistically significant higher WNT7A mRNA expression was observed compared with eutopic endometrium. Expression of WNT7A was found in the luminal and glandular epithelial cells as well as stroma cells in endometrium and endometriosis by immunofluorescence, in situ hybridization, and polymerase chain reaction of laser microdissected tissue. Conclusion(s): The results of the present study suggest that WNT7A plays a role in the pathophysiology of endometriosis.
UR - http://www.scopus.com/inward/record.url?scp=36749000598&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2007.01.128
DO - 10.1016/j.fertnstert.2007.01.128
M3 - Journal articles
C2 - 17588571
AN - SCOPUS:36749000598
SN - 0015-0282
VL - 88
SP - 1534
EP - 1540
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 6
ER -
