TY - JOUR
T1 - Characterization of multidrug-resistance phenotypes and genotypes of Escherichia coli strains isolated from swine from an abattoir in Osaka, Japan
AU - Kumai, Yuko
AU - Suzuki, Yasuhiko
AU - Tanaka, Yoshinori
AU - Shima, Kensuke
AU - Bhadra, Rupak K.
AU - Yamasaki, Shinji
AU - Kuroda, Koichi
AU - Endo, Ginji
PY - 2005/2/1
Y1 - 2005/2/1
N2 - A total of 455 highly tetracycline-resistant Escherichia coli strains were isolated from 84 healthy swine from abattoirs and it was found that 56.9, 43.1, 22.2, 15.4, 2.6 and 1.5% of strains were resistant to chloramphenicol, ampicillin, kanamycin, trimethoprim-sulphamethoxazole, ofloxacin and gentamicin respectively. Interestingly, E. coli strains isolated from certain finisher hog groups exhibited resistance against 2-7 antimicrobials, but strains isolated from multiparous sow groups in each herd were resistant to only 2-4 antimicrobial agents. When randomly selected 108 tetracycline-resistant isolates were tested for the presence of resistance genes, the following genes tet(A) (n=6), tet(B) (n=95), tet(D) (n=1) or both tet(A) and tet(B) (n=6) were found to be distributed among them. Furthermore, 52 isolates carried the integrase 1 gene and 24 strains gave five different PCR amplicon profiles using primers from the variable region of integron. Extensive nucleotide sequence analyses of these amplicons revealed the presence of dhfrI, dhfrXII, dfr17, aadA, aadA2, aadA5, aadA21, aacA4 and catB3 genes which code for different antibacterial resistance proteins.
AB - A total of 455 highly tetracycline-resistant Escherichia coli strains were isolated from 84 healthy swine from abattoirs and it was found that 56.9, 43.1, 22.2, 15.4, 2.6 and 1.5% of strains were resistant to chloramphenicol, ampicillin, kanamycin, trimethoprim-sulphamethoxazole, ofloxacin and gentamicin respectively. Interestingly, E. coli strains isolated from certain finisher hog groups exhibited resistance against 2-7 antimicrobials, but strains isolated from multiparous sow groups in each herd were resistant to only 2-4 antimicrobial agents. When randomly selected 108 tetracycline-resistant isolates were tested for the presence of resistance genes, the following genes tet(A) (n=6), tet(B) (n=95), tet(D) (n=1) or both tet(A) and tet(B) (n=6) were found to be distributed among them. Furthermore, 52 isolates carried the integrase 1 gene and 24 strains gave five different PCR amplicon profiles using primers from the variable region of integron. Extensive nucleotide sequence analyses of these amplicons revealed the presence of dhfrI, dhfrXII, dfr17, aadA, aadA2, aadA5, aadA21, aacA4 and catB3 genes which code for different antibacterial resistance proteins.
UR - http://www.scopus.com/inward/record.url?scp=14044250735&partnerID=8YFLogxK
U2 - 10.1017/S0950268804003280
DO - 10.1017/S0950268804003280
M3 - Journal articles
C2 - 15724712
AN - SCOPUS:14044250735
SN - 0950-2688
VL - 133
SP - 59
EP - 70
JO - Epidemiology and Infection
JF - Epidemiology and Infection
IS - 1
ER -
