TY - JOUR
T1 - Characterization of cDNA coding for the complete light meromyosin portion of a rabbit fast skeletal muscle myosin heavy chain
AU - MAEDA, Kayo
AU - SCZAKIEL, Georg
AU - WITTINGHOFER, Alfred
PY - 1987/8
Y1 - 1987/8
N2 - Myosin‐heavy‐chain‐specific cDNA clones have been isolated from a cDNA library prepared from hind leg muscle of a 14‐day‐old rabbit. According to restriction enzyme analysis these can be grouped into at least two, probably three different classes. RNA dot‐blot hybridization shows that all of these clones correspond to mRNAs expressed in fast skeletal muscle. The clones of the most abundant form, class I, can be aligned to cover the complete light meromyosin portion of myosin heavy chain. The sequence of the coding and the 3′‐untranslated region, together comprising 2143 base pairs, has been determined. The class I clone detects a multigene family of 8–12 members on a Southern blot of rabbit genomic DNA.
AB - Myosin‐heavy‐chain‐specific cDNA clones have been isolated from a cDNA library prepared from hind leg muscle of a 14‐day‐old rabbit. According to restriction enzyme analysis these can be grouped into at least two, probably three different classes. RNA dot‐blot hybridization shows that all of these clones correspond to mRNAs expressed in fast skeletal muscle. The clones of the most abundant form, class I, can be aligned to cover the complete light meromyosin portion of myosin heavy chain. The sequence of the coding and the 3′‐untranslated region, together comprising 2143 base pairs, has been determined. The class I clone detects a multigene family of 8–12 members on a Southern blot of rabbit genomic DNA.
UR - http://www.scopus.com/inward/record.url?scp=0023658390&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1987.tb13308.x
DO - 10.1111/j.1432-1033.1987.tb13308.x
M3 - Journal articles
C2 - 3305014
AN - SCOPUS:0023658390
SN - 0014-2956
VL - 167
SP - 97
EP - 102
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 1
ER -
