Abstract
The anaphylatoxins (AT) C3a and C5a are effector molecules of C3 and C5 exerting multiple biologic functions through binding and activation of their cognate G protein−coupled receptors. C3a interacts with the C3a receptor (C3aR), whereas C5a and its primary degradation product C5a-desArg engage C5aR1 and C5aR2. In the past, analysis of AT expression has been hampered by cross reaction of antibodies designed to recognize the different AT receptors. Furthermore, assessment of effects mediated by cell-specific activation has been difficult. Here, floxed AT receptor reporter mice are described as tools to monitor AT receptor expression in cells and tissues and to study the functions of C3a and C5a by cell-specific deletion of their cognate AT receptors.
| Original language | English |
|---|---|
| Article number | e100 |
| Journal | Current Protocols in Immunology |
| Volume | 130 |
| Issue number | 1 |
| ISSN | 1934-3671 |
| DOIs | |
| Publication status | Published - 01.09.2020 |
Funding
Open access funding enabled and organized by Projekt DEAL. This work was supported by funds from the German Research Foundation (DFG) for the International Research Training Group (IRTG) 1911 (projects A1, A7, B1, and B2 to Y.L., C.M.K., and J.K.), as well as the EXC 306 and EXC 2167 to J.K. We thank Katharina Quell and Katharina Bröker for help in establishing the functional AT receptor assays and T. Vollbrandt for his comments regarding the flow cytometer settings.
Research Areas and Centers
- Academic Focus: Center for Infection and Inflammation Research (ZIEL)
