Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef

H. S. Kumar; Indrani Karunasagar; I. Karunasagar; Tsukamoto Teizou; Kensuke Shima; Shinji Yamasaki

doi:10.1016/j.femsle.2004.02.008

Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef

H. S. Kumar, Indrani Karunasagar, I. Karunasagar, Tsukamoto Teizou, Kensuke Shima, Shinji Yamasaki

Institute of Medical Microbiology

56 Citations (Scopus)

Abstract

Shiga toxin-producing Escherichia coli (STEC) strains isolated in Mangalore, India, were characterised by bead-enzyme-linked immunosorbent assay (bead-ELISA), Vero cell cytotoxicity assay, PCR and colony hybridisation for the detection of stx₁ and stx₂ genes. Four strains from seafood, six from beef and one from a clinical case of bloody diarrhoea were positive for Shiga toxins Stx1 and Stx2 and also for stx₁ and stx₂ genes. The seafood isolates produced either Stx2 alone or both Stx1 and Stx2, while the beef isolates produced Stx1 alone. The stx₁ gene of all the beef STEC was found to be of recently reported stx₁c type. All STEC strains and one non-STEC strain isolated from clam harboured EHEC-hlyA. Interestingly, though all STEC strains were negative for eae gene, two STEC strains isolated from seafood and one from a patient with bloody diarrhoea possessed STEC autoagglutinating adhesion (saa) gene, recently identified as a gene encoding a novel autoagglutinating adhesion.

Original language	English
Journal	FEMS Microbiology Letters
Volume	233
Issue number	1
Pages (from-to)	173-178
Number of pages	6
ISSN	0378-1097
DOIs	https://doi.org/10.1016/j.femsle.2004.02.008
Publication status	Published - 01.04.2004

Funding

H.S.K. was a recipient of UNESCO fellowship for young scientist. The work in India on STEC was done under the Indo-German collaborative project.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

Access to Document

10.1016/j.femsle.2004.02.008

Cite this

@article{fc86d19fea3748919aa97da73b25d527,

title = "Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef",

abstract = "Shiga toxin-producing Escherichia coli (STEC) strains isolated in Mangalore, India, were characterised by bead-enzyme-linked immunosorbent assay (bead-ELISA), Vero cell cytotoxicity assay, PCR and colony hybridisation for the detection of stx1 and stx2 genes. Four strains from seafood, six from beef and one from a clinical case of bloody diarrhoea were positive for Shiga toxins Stx1 and Stx2 and also for stx1 and stx2 genes. The seafood isolates produced either Stx2 alone or both Stx1 and Stx2, while the beef isolates produced Stx1 alone. The stx1 gene of all the beef STEC was found to be of recently reported stx1c type. All STEC strains and one non-STEC strain isolated from clam harboured EHEC-hlyA. Interestingly, though all STEC strains were negative for eae gene, two STEC strains isolated from seafood and one from a patient with bloody diarrhoea possessed STEC autoagglutinating adhesion (saa) gene, recently identified as a gene encoding a novel autoagglutinating adhesion.",

author = "Kumar, \{H. S.\} and Indrani Karunasagar and I. Karunasagar and Tsukamoto Teizou and Kensuke Shima and Shinji Yamasaki",

year = "2004",

month = apr,

day = "1",

doi = "10.1016/j.femsle.2004.02.008",

language = "English",

volume = "233",

pages = "173--178",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef

AU - Kumar, H. S.

AU - Karunasagar, Indrani

AU - Karunasagar, I.

AU - Teizou, Tsukamoto

AU - Shima, Kensuke

AU - Yamasaki, Shinji

PY - 2004/4/1

Y1 - 2004/4/1

N2 - Shiga toxin-producing Escherichia coli (STEC) strains isolated in Mangalore, India, were characterised by bead-enzyme-linked immunosorbent assay (bead-ELISA), Vero cell cytotoxicity assay, PCR and colony hybridisation for the detection of stx1 and stx2 genes. Four strains from seafood, six from beef and one from a clinical case of bloody diarrhoea were positive for Shiga toxins Stx1 and Stx2 and also for stx1 and stx2 genes. The seafood isolates produced either Stx2 alone or both Stx1 and Stx2, while the beef isolates produced Stx1 alone. The stx1 gene of all the beef STEC was found to be of recently reported stx1c type. All STEC strains and one non-STEC strain isolated from clam harboured EHEC-hlyA. Interestingly, though all STEC strains were negative for eae gene, two STEC strains isolated from seafood and one from a patient with bloody diarrhoea possessed STEC autoagglutinating adhesion (saa) gene, recently identified as a gene encoding a novel autoagglutinating adhesion.

AB - Shiga toxin-producing Escherichia coli (STEC) strains isolated in Mangalore, India, were characterised by bead-enzyme-linked immunosorbent assay (bead-ELISA), Vero cell cytotoxicity assay, PCR and colony hybridisation for the detection of stx1 and stx2 genes. Four strains from seafood, six from beef and one from a clinical case of bloody diarrhoea were positive for Shiga toxins Stx1 and Stx2 and also for stx1 and stx2 genes. The seafood isolates produced either Stx2 alone or both Stx1 and Stx2, while the beef isolates produced Stx1 alone. The stx1 gene of all the beef STEC was found to be of recently reported stx1c type. All STEC strains and one non-STEC strain isolated from clam harboured EHEC-hlyA. Interestingly, though all STEC strains were negative for eae gene, two STEC strains isolated from seafood and one from a patient with bloody diarrhoea possessed STEC autoagglutinating adhesion (saa) gene, recently identified as a gene encoding a novel autoagglutinating adhesion.

UR - https://www.scopus.com/pages/publications/1642390795

U2 - 10.1016/j.femsle.2004.02.008

DO - 10.1016/j.femsle.2004.02.008

M3 - Journal articles

C2 - 15043885

AN - SCOPUS:1642390795

SN - 0378-1097

VL - 233

SP - 173

EP - 178

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef

Abstract

Funding

UN SDGs

Research Areas and Centers

Access to Document

Other files and links

Fingerprint

Cite this