Changes in human hepatic metabolism in steatosis and cirrhosis

Zoe Schofield; Michelle A.C. Reed; Philip N. Newsome; David H. Adams; Ulrich L. Günther; Patricia F. Lalor

doi:10.3748/wjg.v23.i15.2685

Changes in human hepatic metabolism in steatosis and cirrhosis

Zoe Schofield, Michelle A.C. Reed, Philip N. Newsome, David H. Adams, Ulrich L. Günther, Patricia F. Lalor^*

^*Corresponding author for this work

Institute of Chemistry and Metabolomics

15 Citations (Scopus)

Abstract

AIM: To understand the underlying metabolic changes in human liver disease we have applied nuclear magnetic resonance (NMR) metabolomics analysis to human liver tissue. METHODS: We have carried out pilot study using ¹H-NMR to derive metabolomic signatures from human liver from patients with steatosis, nonalcoholic steatohepatitis (NASH) or alcohol-related liver damage (ARLD) to identify species that can predict outcome and discriminate between alcohol and metabolic-induced liver injuries. RESULTS: Changes in branched chain amino acid homeostasis, tricarboxylic acid cycle and purine biosynthesis intermediates along with betaine were associated with the development of cirrhosis in both ARLD and nonalcoholic fatty liver disease. Species such as propylene glycol and as yet unidentified moieties that allowed discrimination between NASH and ARLD samples were also detected using our approach. CONCLUSION: Our high throughput, non-destructive technique for multiple analyte quantification in human liver specimens has potential for identification of biomarkers with prognostic and diagnostic significance.

Original language	English
Journal	World Journal of Gastroenterology
Volume	23
Issue number	15
Pages (from-to)	2685-2695
Number of pages	11
ISSN	1007-9327
DOIs	https://doi.org/10.3748/wjg.v23.i15.2685
Publication status	Published - 21.04.2017

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Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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10.3748/wjg.v23.i15.2685

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title = "Changes in human hepatic metabolism in steatosis and cirrhosis",

abstract = "AIM: To understand the underlying metabolic changes in human liver disease we have applied nuclear magnetic resonance (NMR) metabolomics analysis to human liver tissue. METHODS: We have carried out pilot study using 1H-NMR to derive metabolomic signatures from human liver from patients with steatosis, nonalcoholic steatohepatitis (NASH) or alcohol-related liver damage (ARLD) to identify species that can predict outcome and discriminate between alcohol and metabolic-induced liver injuries. RESULTS: Changes in branched chain amino acid homeostasis, tricarboxylic acid cycle and purine biosynthesis intermediates along with betaine were associated with the development of cirrhosis in both ARLD and nonalcoholic fatty liver disease. Species such as propylene glycol and as yet unidentified moieties that allowed discrimination between NASH and ARLD samples were also detected using our approach. CONCLUSION: Our high throughput, non-destructive technique for multiple analyte quantification in human liver specimens has potential for identification of biomarkers with prognostic and diagnostic significance.",

author = "Zoe Schofield and Reed, {Michelle A.C.} and Newsome, {Philip N.} and Adams, {David H.} and G{\"u}nther, {Ulrich L.} and Lalor, {Patricia F.}",

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T1 - Changes in human hepatic metabolism in steatosis and cirrhosis

AU - Schofield, Zoe

AU - Reed, Michelle A.C.

AU - Newsome, Philip N.

AU - Adams, David H.

AU - Günther, Ulrich L.

AU - Lalor, Patricia F.

PY - 2017/4/21

Y1 - 2017/4/21

N2 - AIM: To understand the underlying metabolic changes in human liver disease we have applied nuclear magnetic resonance (NMR) metabolomics analysis to human liver tissue. METHODS: We have carried out pilot study using 1H-NMR to derive metabolomic signatures from human liver from patients with steatosis, nonalcoholic steatohepatitis (NASH) or alcohol-related liver damage (ARLD) to identify species that can predict outcome and discriminate between alcohol and metabolic-induced liver injuries. RESULTS: Changes in branched chain amino acid homeostasis, tricarboxylic acid cycle and purine biosynthesis intermediates along with betaine were associated with the development of cirrhosis in both ARLD and nonalcoholic fatty liver disease. Species such as propylene glycol and as yet unidentified moieties that allowed discrimination between NASH and ARLD samples were also detected using our approach. CONCLUSION: Our high throughput, non-destructive technique for multiple analyte quantification in human liver specimens has potential for identification of biomarkers with prognostic and diagnostic significance.

AB - AIM: To understand the underlying metabolic changes in human liver disease we have applied nuclear magnetic resonance (NMR) metabolomics analysis to human liver tissue. METHODS: We have carried out pilot study using 1H-NMR to derive metabolomic signatures from human liver from patients with steatosis, nonalcoholic steatohepatitis (NASH) or alcohol-related liver damage (ARLD) to identify species that can predict outcome and discriminate between alcohol and metabolic-induced liver injuries. RESULTS: Changes in branched chain amino acid homeostasis, tricarboxylic acid cycle and purine biosynthesis intermediates along with betaine were associated with the development of cirrhosis in both ARLD and nonalcoholic fatty liver disease. Species such as propylene glycol and as yet unidentified moieties that allowed discrimination between NASH and ARLD samples were also detected using our approach. CONCLUSION: Our high throughput, non-destructive technique for multiple analyte quantification in human liver specimens has potential for identification of biomarkers with prognostic and diagnostic significance.

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SN - 1007-9327

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JO - World Journal of Gastroenterology

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Changes in human hepatic metabolism in steatosis and cirrhosis

Abstract

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