Cellular localization and expression of the serotonin transporter in mouse brain

D Bengel; O Jöhren; A M Andrews; A Heils; R Mössner; G L Sanvitto; J M Saavedra; K P Lesch; D L Murphy

doi:10.1016/s0006-8993(97)01080-9

Cellular localization and expression of the serotonin transporter in mouse brain

D Bengel, O Jöhren, A M Andrews, A Heils, R Mössner, G L Sanvitto, J M Saavedra, K P Lesch, D L Murphy

Institute of Experimental and Clinical Pharmacology and Toxicology

National Institutes of Health

Abstract

The high-affinity serotonin (5-HT) transporter (5-HTT) plays an important role in the removal of extracellular serotonin, thereby modulating and terminating the action of this neurotransmitter at various pre- and post-synaptic serotonergic receptors and heteroreceptors. In order to characterize the anatomical distribution of the 5-HTT in mouse brain, in situ hybridization histochemistry using 35S-labeled riboprobes was performed. These results were compared with 5-HTT binding site distribution as evaluated by [125I]RTI-55 autoradiography. High levels of 5-HTT mRNA were detected in all brain stem raphe nuclei, with variations in labeling among the various subnuclei. Those brain areas known to possess serotonergic cell bodies stained intensely for both 5-HTT mRNA and 5-HTT binding sites. In contrast to previous findings in rat brain, the highest densities of 5-HTT sites were found in areas outside the raphe complex, particularly in the substantia nigra, globus pallidus, and superior colliculi.

Original language	English
Journal	Brain Research
Volume	778
Issue number	2
Pages (from-to)	338-45
Number of pages	8
ISSN	0006-8993
DOIs	https://doi.org/10.1016/s0006-8993(97)01080-9
Publication status	Published - 1997

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10.1016/s0006-8993(97)01080-9

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title = "Cellular localization and expression of the serotonin transporter in mouse brain",

abstract = "The high-affinity serotonin (5-HT) transporter (5-HTT) plays an important role in the removal of extracellular serotonin, thereby modulating and terminating the action of this neurotransmitter at various pre- and post-synaptic serotonergic receptors and heteroreceptors. In order to characterize the anatomical distribution of the 5-HTT in mouse brain, in situ hybridization histochemistry using 35S-labeled riboprobes was performed. These results were compared with 5-HTT binding site distribution as evaluated by [125I]RTI-55 autoradiography. High levels of 5-HTT mRNA were detected in all brain stem raphe nuclei, with variations in labeling among the various subnuclei. Those brain areas known to possess serotonergic cell bodies stained intensely for both 5-HTT mRNA and 5-HTT binding sites. In contrast to previous findings in rat brain, the highest densities of 5-HTT sites were found in areas outside the raphe complex, particularly in the substantia nigra, globus pallidus, and superior colliculi.",

author = "D Bengel and O J{\"o}hren and Andrews, {A M} and A Heils and R M{\"o}ssner and Sanvitto, {G L} and Saavedra, {J M} and Lesch, {K P} and Murphy, {D L}",

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T1 - Cellular localization and expression of the serotonin transporter in mouse brain

AU - Bengel, D

AU - Jöhren, O

AU - Andrews, A M

AU - Heils, A

AU - Mössner, R

AU - Sanvitto, G L

AU - Saavedra, J M

AU - Lesch, K P

AU - Murphy, D L

PY - 1997

Y1 - 1997

N2 - The high-affinity serotonin (5-HT) transporter (5-HTT) plays an important role in the removal of extracellular serotonin, thereby modulating and terminating the action of this neurotransmitter at various pre- and post-synaptic serotonergic receptors and heteroreceptors. In order to characterize the anatomical distribution of the 5-HTT in mouse brain, in situ hybridization histochemistry using 35S-labeled riboprobes was performed. These results were compared with 5-HTT binding site distribution as evaluated by [125I]RTI-55 autoradiography. High levels of 5-HTT mRNA were detected in all brain stem raphe nuclei, with variations in labeling among the various subnuclei. Those brain areas known to possess serotonergic cell bodies stained intensely for both 5-HTT mRNA and 5-HTT binding sites. In contrast to previous findings in rat brain, the highest densities of 5-HTT sites were found in areas outside the raphe complex, particularly in the substantia nigra, globus pallidus, and superior colliculi.

AB - The high-affinity serotonin (5-HT) transporter (5-HTT) plays an important role in the removal of extracellular serotonin, thereby modulating and terminating the action of this neurotransmitter at various pre- and post-synaptic serotonergic receptors and heteroreceptors. In order to characterize the anatomical distribution of the 5-HTT in mouse brain, in situ hybridization histochemistry using 35S-labeled riboprobes was performed. These results were compared with 5-HTT binding site distribution as evaluated by [125I]RTI-55 autoradiography. High levels of 5-HTT mRNA were detected in all brain stem raphe nuclei, with variations in labeling among the various subnuclei. Those brain areas known to possess serotonergic cell bodies stained intensely for both 5-HTT mRNA and 5-HTT binding sites. In contrast to previous findings in rat brain, the highest densities of 5-HTT sites were found in areas outside the raphe complex, particularly in the substantia nigra, globus pallidus, and superior colliculi.

U2 - 10.1016/s0006-8993(97)01080-9

DO - 10.1016/s0006-8993(97)01080-9

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SN - 0006-8993

VL - 778

SP - 338

EP - 345

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Cellular localization and expression of the serotonin transporter in mouse brain

Abstract

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