CdSe/CdS-quantum rods: Fluorescent probes for in vivo two-photon laser scanning microscopy

Jelena Dimitrijevic; Lisa Krapf; Christopher Wolter; Christian Schmidtke; Jan Philip Merkl; Tobias Jochum; Andreas Kornowski; Anna Schüth; Andreas Gebert; Gereon Hüttmann; Tobias Vossmeyer; Horst Weller

doi:10.1039/c4nr02702g

CdSe/CdS-quantum rods: Fluorescent probes for in vivo two-photon laser scanning microscopy

Jelena Dimitrijevic, Lisa Krapf, Christopher Wolter, Christian Schmidtke, Jan Philip Merkl, Tobias Jochum, Andreas Kornowski, Anna Schüth, Andreas Gebert, Gereon Hüttmann, Tobias Vossmeyer^*, Horst Weller

^*Corresponding author for this work

University of Hamburg

31 Citations (Scopus)

Abstract

CdSe/CdS-Quantum-dots-quantum-rods (QDQRs) with an aspect ratio of ∼6 are prepared via the seeded growth method, encapsulated within a shell of crosslinked poly(isoprene)-block-poly(ethylene glycol) (PI-b-PEG) diblock copolymer, and transferred from the organic phase into aqueous media. Their photoluminescence quantum yield (PLQY) of 78% is not compromised by the phase transfer. Within a period of two months the PLQY of QDQRs in aqueous solution at neutral pH decreases only slightly (to ∼65%). The two-photon (TP) action cross sections of QDQRs (∼10⁵ GM) are two orders of magnitude higher than those of CdSe/CdS/ZnS-core/shell/shell quantum dots (QDs, ∼10³ GM) with comparable diameter (∼5 nm). After applying PI-b-PEG encapsulated QDQRs onto the small intestinal mucosa of mice in vivo, their strong red fluorescence can easily be observed by two-photon laser scanning microscopy (TPLSM) and clearly distinguished from autofluorescent background. Our results demonstrate that PI-b-PEG encapsulated CdSe/CdS-QDQRs are excellent probes for studying the uptake and fate of nanoparticles by two-photon imaging techniques in vivo.

Original language	English
Journal	Nanoscale
Volume	6
Issue number	17
Pages (from-to)	10413-10422
Number of pages	10
ISSN	2040-3364
DOIs	https://doi.org/10.1039/c4nr02702g
Publication status	Published - 07.09.2014

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Academic Focus: Biomedical Engineering

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title = "CdSe/CdS-quantum rods: Fluorescent probes for in vivo two-photon laser scanning microscopy",

abstract = "CdSe/CdS-Quantum-dots-quantum-rods (QDQRs) with an aspect ratio of ∼6 are prepared via the seeded growth method, encapsulated within a shell of crosslinked poly(isoprene)-block-poly(ethylene glycol) (PI-b-PEG) diblock copolymer, and transferred from the organic phase into aqueous media. Their photoluminescence quantum yield (PLQY) of 78% is not compromised by the phase transfer. Within a period of two months the PLQY of QDQRs in aqueous solution at neutral pH decreases only slightly (to ∼65%). The two-photon (TP) action cross sections of QDQRs (∼105 GM) are two orders of magnitude higher than those of CdSe/CdS/ZnS-core/shell/shell quantum dots (QDs, ∼103 GM) with comparable diameter (∼5 nm). After applying PI-b-PEG encapsulated QDQRs onto the small intestinal mucosa of mice in vivo, their strong red fluorescence can easily be observed by two-photon laser scanning microscopy (TPLSM) and clearly distinguished from autofluorescent background. Our results demonstrate that PI-b-PEG encapsulated CdSe/CdS-QDQRs are excellent probes for studying the uptake and fate of nanoparticles by two-photon imaging techniques in vivo.",

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AU - Dimitrijevic, Jelena

AU - Krapf, Lisa

AU - Wolter, Christopher

AU - Schmidtke, Christian

AU - Merkl, Jan Philip

AU - Jochum, Tobias

AU - Kornowski, Andreas

AU - Schüth, Anna

AU - Gebert, Andreas

AU - Hüttmann, Gereon

AU - Vossmeyer, Tobias

AU - Weller, Horst

PY - 2014/9/7

Y1 - 2014/9/7

N2 - CdSe/CdS-Quantum-dots-quantum-rods (QDQRs) with an aspect ratio of ∼6 are prepared via the seeded growth method, encapsulated within a shell of crosslinked poly(isoprene)-block-poly(ethylene glycol) (PI-b-PEG) diblock copolymer, and transferred from the organic phase into aqueous media. Their photoluminescence quantum yield (PLQY) of 78% is not compromised by the phase transfer. Within a period of two months the PLQY of QDQRs in aqueous solution at neutral pH decreases only slightly (to ∼65%). The two-photon (TP) action cross sections of QDQRs (∼105 GM) are two orders of magnitude higher than those of CdSe/CdS/ZnS-core/shell/shell quantum dots (QDs, ∼103 GM) with comparable diameter (∼5 nm). After applying PI-b-PEG encapsulated QDQRs onto the small intestinal mucosa of mice in vivo, their strong red fluorescence can easily be observed by two-photon laser scanning microscopy (TPLSM) and clearly distinguished from autofluorescent background. Our results demonstrate that PI-b-PEG encapsulated CdSe/CdS-QDQRs are excellent probes for studying the uptake and fate of nanoparticles by two-photon imaging techniques in vivo.

AB - CdSe/CdS-Quantum-dots-quantum-rods (QDQRs) with an aspect ratio of ∼6 are prepared via the seeded growth method, encapsulated within a shell of crosslinked poly(isoprene)-block-poly(ethylene glycol) (PI-b-PEG) diblock copolymer, and transferred from the organic phase into aqueous media. Their photoluminescence quantum yield (PLQY) of 78% is not compromised by the phase transfer. Within a period of two months the PLQY of QDQRs in aqueous solution at neutral pH decreases only slightly (to ∼65%). The two-photon (TP) action cross sections of QDQRs (∼105 GM) are two orders of magnitude higher than those of CdSe/CdS/ZnS-core/shell/shell quantum dots (QDs, ∼103 GM) with comparable diameter (∼5 nm). After applying PI-b-PEG encapsulated QDQRs onto the small intestinal mucosa of mice in vivo, their strong red fluorescence can easily be observed by two-photon laser scanning microscopy (TPLSM) and clearly distinguished from autofluorescent background. Our results demonstrate that PI-b-PEG encapsulated CdSe/CdS-QDQRs are excellent probes for studying the uptake and fate of nanoparticles by two-photon imaging techniques in vivo.

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CdSe/CdS-quantum rods: Fluorescent probes for in vivo two-photon laser scanning microscopy

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