Cancer cell-specific protein delivery by optoporation with laser-irradiated gold nanorods

Cuiping Yao; Florian Rudnitzki; Yida He; Zhenxi Zhang; Gereon Hüttmann; Ramtin Rahmanzadeh

doi:10.1002/jbio.202000017

Cancer cell-specific protein delivery by optoporation with laser-irradiated gold nanorods

Cuiping Yao, Florian Rudnitzki, Yida He, Zhenxi Zhang, Gereon Hüttmann, Ramtin Rahmanzadeh^*

^*Corresponding author for this work

Xi'an Jiaotong University

24 Citations (Scopus)

Abstract

The delivery of macromolecules into living cells is challenging since in most cases molecules are endocytosed and remain in the endo-lysosomal pathway where they are degraded before reaching their target. Here, a method is presented to selectively improve cell membrane permeability by nanosecond laser irradiation of gold nanorods (GNRs) with visible or near-infrared irradiation in order to deliver proteins across the plasma membrane, avoiding the endo lysosomal pathway. GNRs were labeled with the anti-EGFR (epidermal growth factor receptor) antibody Erbitux to target human ovarian carcinoma cells OVCAR-3. Irradiation with nanosecond laser pulses at wavelengths of 532 nm or 730 nm is used for transient permeabilization of the cell membranes. As a result of the irradiation, the uptake of an anti-Ki-67 antibody was observed in about 50 % of the cells. The results of fluorescence lifetime imaging show that the GNR detached from the membrane after irradiation.

Original language	English
Article number	e202000017
Journal	Journal of Biophotonics
Volume	13
Issue number	7
ISSN	1864-063X
DOIs	https://doi.org/10.1002/jbio.202000017
Publication status	Published - 01.07.2020

Funding

We acknowledge Barbara Flucke and Astrid Link for their assistance during this study. This work was supported by the German Ministry of Education and Research (grant no. 13N11832), the German Research Foundation (DFG, grant no. Ra1771/4‐1), the European Union project ENCOMOLE‐2i (Horizon 2020, ERC CoG 646669) and the National Natural Science Foundation of China under grants 61775178 and 61727823. And the Fundamental Research Funds for the Central Universities zdyf2017010.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 9 Industry, Innovation, and Infrastructure

Research Areas and Centers

Academic Focus: Biomedical Engineering

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10.1002/jbio.202000017

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title = "Cancer cell-specific protein delivery by optoporation with laser-irradiated gold nanorods",

abstract = "The delivery of macromolecules into living cells is challenging since in most cases molecules are endocytosed and remain in the endo-lysosomal pathway where they are degraded before reaching their target. Here, a method is presented to selectively improve cell membrane permeability by nanosecond laser irradiation of gold nanorods (GNRs) with visible or near-infrared irradiation in order to deliver proteins across the plasma membrane, avoiding the endo lysosomal pathway. GNRs were labeled with the anti-EGFR (epidermal growth factor receptor) antibody Erbitux to target human ovarian carcinoma cells OVCAR-3. Irradiation with nanosecond laser pulses at wavelengths of 532 nm or 730 nm is used for transient permeabilization of the cell membranes. As a result of the irradiation, the uptake of an anti-Ki-67 antibody was observed in about 50 \% of the cells. The results of fluorescence lifetime imaging show that the GNR detached from the membrane after irradiation.",

author = "Cuiping Yao and Florian Rudnitzki and Yida He and Zhenxi Zhang and Gereon H{\"u}ttmann and Ramtin Rahmanzadeh",

note = "Funding Information: We acknowledge Barbara Flucke and Astrid Link for their assistance during this study. This work was supported by the German Ministry of Education and Research (grant no. 13N11832), the German Research Foundation (DFG, grant no. Ra1771/4‐1), the European Union project ENCOMOLE‐2i (Horizon 2020, ERC CoG 646669) and the National Natural Science Foundation of China under grants 61775178 and 61727823. And the Fundamental Research Funds for the Central Universities zdyf2017010. Publisher Copyright: {\textcopyright} 2020 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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doi = "10.1002/jbio.202000017",

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AU - Yao, Cuiping

AU - Rudnitzki, Florian

AU - He, Yida

AU - Zhang, Zhenxi

AU - Hüttmann, Gereon

AU - Rahmanzadeh, Ramtin

N1 - Funding Information: We acknowledge Barbara Flucke and Astrid Link for their assistance during this study. This work was supported by the German Ministry of Education and Research (grant no. 13N11832), the German Research Foundation (DFG, grant no. Ra1771/4‐1), the European Union project ENCOMOLE‐2i (Horizon 2020, ERC CoG 646669) and the National Natural Science Foundation of China under grants 61775178 and 61727823. And the Fundamental Research Funds for the Central Universities zdyf2017010. Publisher Copyright: © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/7/1

Y1 - 2020/7/1

N2 - The delivery of macromolecules into living cells is challenging since in most cases molecules are endocytosed and remain in the endo-lysosomal pathway where they are degraded before reaching their target. Here, a method is presented to selectively improve cell membrane permeability by nanosecond laser irradiation of gold nanorods (GNRs) with visible or near-infrared irradiation in order to deliver proteins across the plasma membrane, avoiding the endo lysosomal pathway. GNRs were labeled with the anti-EGFR (epidermal growth factor receptor) antibody Erbitux to target human ovarian carcinoma cells OVCAR-3. Irradiation with nanosecond laser pulses at wavelengths of 532 nm or 730 nm is used for transient permeabilization of the cell membranes. As a result of the irradiation, the uptake of an anti-Ki-67 antibody was observed in about 50 % of the cells. The results of fluorescence lifetime imaging show that the GNR detached from the membrane after irradiation.

AB - The delivery of macromolecules into living cells is challenging since in most cases molecules are endocytosed and remain in the endo-lysosomal pathway where they are degraded before reaching their target. Here, a method is presented to selectively improve cell membrane permeability by nanosecond laser irradiation of gold nanorods (GNRs) with visible or near-infrared irradiation in order to deliver proteins across the plasma membrane, avoiding the endo lysosomal pathway. GNRs were labeled with the anti-EGFR (epidermal growth factor receptor) antibody Erbitux to target human ovarian carcinoma cells OVCAR-3. Irradiation with nanosecond laser pulses at wavelengths of 532 nm or 730 nm is used for transient permeabilization of the cell membranes. As a result of the irradiation, the uptake of an anti-Ki-67 antibody was observed in about 50 % of the cells. The results of fluorescence lifetime imaging show that the GNR detached from the membrane after irradiation.

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ER -

Cancer cell-specific protein delivery by optoporation with laser-irradiated gold nanorods

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